Coumarin-Based Reversible Fluorescent Probe for Selective Detection of Cu2+ in Living Cells.

Journal of Fluorescence
Nadeem AhmedYong Ye

Abstract

Copper ion plays an important role in many biological processes in human body. H2S is considered as the third gasses transmitter after carbon monoxide and nitric oxide. Here a novel ICT-based fluorescent ON-OFF-ON probe for Cu2+ and H2S detection was developed. Selectivity and sensitivity of probe was confirmed in aqueous Tris-HCl buffer (10 mM, pH 7.4, containing 90% acetonitrile). Probe DF-CU shows high selectivity over other analytes. The degree of fluorescence quenching is linearly associated with the concentration of Cu2+ (R2 = 0.9919). The limit of detection (LOD, calculated according to the 3σ/slope) for Cu2+ was 6.4 μM. Probe can work in almost all pH. The probe shows a very fast response to Cu2+ (within 10 s). Its response to copper ion could be reversed by H2S. The complex of probe with Cu2+ could be used for H2S detection. Furthermore, this ON-OFF-ON fluorescent probe successfully applied in the living cells for the detection of Cu2+ and H2S.

References

May 19, 2000·The Journal of Biological Chemistry·T V O'Halloran, V C Culotta
Oct 24, 2002·Molecular Neurobiology·Hideo Kimura
Jan 24, 2003·Journal of Bioenergetics and Biomembranes·Diane W Cox, Steven D P Moore
Feb 16, 2007·Current Opinion in Chemical Biology·Paul S DonnellyAnthony G Wedd
Oct 20, 2007·Nature Reviews. Drug Discovery·Csaba Szabó
Jul 18, 2009·Circulation Research·John W CalvertDavid J Lefer
Jan 8, 2011·Annual Review of Pharmacology and Toxicology·Ling LiPhilip K Moore
Jan 12, 2011·Nature Reviews. Neurology·Stephen G Kaler
Feb 2, 2011·Chemical Communications : Chem Comm·Kyoung Chul KoJong Seung Kim
Aug 31, 2011·Organic Letters·Hyo Sung JungJong Seung Kim
Feb 20, 2015·Chemical Society Reviews·Brian DalyA Prasanna de Silva

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Citations

Jun 12, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Jianhua WeiZhonghai Ni
Sep 3, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Nadeem AhmedYong Ye

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Methods Mentioned

BETA
fluorescence imaging
column chromatography

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