PMID: 16526407Mar 11, 2006Paper

Counter-selectable marker for bacterial-based interaction trap systems

BioTechniques
Xiangdong MengScot A Wolfe

Abstract

Counter-selectable markers can be used in two-hybrid systems to search libraries for a protein or compound that interferes with a macromolecular interaction or to identify macromolecules from a population that cannot mediate a particular interaction. In this report, we describe the adaptation of the yeast URA3/5-FOA counter-selection system for use in bacterial interaction trap experiments. Two different URA3 reporter systems were developed that allow robust counter-selection: (i) a single copy F' episome reporter and (ii) a co-cistronic HIS3-URA3 reporter vector. The HIS3-URA3 reporter can be used for either positive or negative selections in appropriate bacterial strains. These reagents extend the utility of the bacterial two-hybrid system as an alternative to its yeast-based counterpart.

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Citations

Nov 19, 2013·Nucleic Acids Research·Trevor Siggers, Raluca Gordân
Nov 29, 2014·Briefings in Functional Genomics·Kellen K AndrilenasTrevor Siggers
Aug 19, 2014·Trends in Biochemical Sciences·Matthew SlatteryRemo Rohs
Mar 19, 2020·Angewandte Chemie·Martin SpinckHeinz Neumann
Dec 24, 2020·Nature Communications·Beatriz ÁlvarezLuis Ángel Fernández

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