Countercurrent chromatographic separation: a hydrodynamic approach developed for extraction columns

Journal of Chromatography. a
A E KostanianIan A Sutherland

Abstract

In countercurrent chromatography (CCC) both stationary and mobile liquids undergo intense mixing in the variable force field of a coil planet centrifuge and the separation process, like the separation in conventional solvent extraction column, is influenced by longitudinal mixing in the phases and mass transfer between them. This paper describes how the residence time distribution (or the elution profile) of a solute in CCC devices and the interpretation of experimental peaks, can be described by a recently developed cell model of longitudinal mixing. The model considers a CCC column as a cascade of perfectly mixed equal-size cells, the number of which is determined by the rates of longitudinal mixing in the stationary and mobile phases. Experiments were carried out to demonstrate the validation of the model and the possibility of predicting the partitioning behaviour of the solutes. The methods for estimating model parameters are discussed. Longitudinal mixing rates in stationary and mobile phases have been experimentally determined and experimental elution profiles are compared with simulated peaks. It is shown that using the cell model the peak shape for a solute with a given distribution constant can be predicted from exper...Continue Reading

References

Nov 7, 2001·Journal of Chromatography. a·V B Di Marco, G G Bombi

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Citations

May 12, 2009·Journal of Chromatography. a·Chun-Xia ZhaoChao-Hong He
Jan 13, 2015·Journal of Liquid Chromatography & Related Technologies·Fengkang WangYun Wei
Sep 15, 2020·Journal of Separation Science·Yuru GuoJizhong Yan
Jul 16, 2013·Journal of Chromatography. a·Peter HewitsonSvetlana Ignatova
Aug 1, 2008·Journal of Natural Products·Guido F PauliJ Brent Friesen

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