Countercurrent chromatographic separation of proteins using an eccentric coiled column with synchronous and nonsynchronous type-J planetary motions

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
Kazufusa ShinomiyaYoichiro Ito

Abstract

Protein separation was performed using the high-speed countercurrent chromatograph (HSCCC) at both synchronous and nonsynchronous type-J planetary motions. The partition efficiency was evaluated with two different column configurations, eccentric coil and toroidal coil, on the separation of a set of stable protein samples including cytochrome C, myoglobin and lysozyme with a polymer phase system composed of 12.5% (w/w) polyethylene glycol 1000 and 12.5% (w/w) dibasic potassium phosphate. Better peak resolution was obtained by the eccentric coil than by the toroidal coil using either lower or upper phase as the mobile phase. The peak resolution was further improved using the eccentric coil by the nonsynchronous type-J planetary motion with the combination of 1066 rpm of column rotation and 1000 rpm of revolution.

References

Apr 2, 2008·Journal of Chromatography. a·I A SutherlandO Intes
Mar 11, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Kazufusa ShinomiyaYoichiro Ito

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Citations

Apr 2, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yuki UtoKoichi Inoue
Oct 27, 2020·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Kazufusa ShinomiyaYoichiro Ito

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