PMID: 7032593Dec 7, 1981Paper

Counterflow efflux of thiamin in Escherichia coli

Biochimica Et Biophysica Acta
T NishimuneR Hayashi

Abstract

A resting cell of Escherichia coli lacking thiamin kinase incorporated external thiamin with an energy-dependent counterflow efflux (C-efflux). This C-efflux could be separated frm an energy-dependent exit by a selective inhibition of exit by 2 x 10(-2) M NaN3. The extracellular thiamin could be replaced by thiamin diphosphate, resulting in the same rate of C-efflux, but the rate of C-efflux of intracellular thiamin diphosphate against the external thiamin was markedly low. This low rate of C-efflux of thiamin diphosphate could explain the higher accumulation of the compound than that of free thiamin in the thiamin-kinase-defective mutant as well as in its wild-type parent. Basic characteristics of free thiamin uptake and exit in E. coli W mutant were compared with those reported in K 12 mutant: a marked difference existed in the rate of exit. The low rate of exit in E. coli W 70-23-102 was inferred as the reason for the absence of an overshoot phenomenon of thiamin uptake in this strain.

References

Apr 1, 1969·Archives of Biochemistry and Biophysics·T KawasakiY Nose
Jun 16, 1999·The Laryngoscope·L F GrymerH Baymler Pedersen

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Citations

Jan 1, 1988·Folia Microbiologica·T RumlP Rauch
Jul 1, 1986·The Journal of Applied Bacteriology·S Ratnam, S B March

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