Jun 1, 1976

Counterimmunoelectrophoresis of staphylococcal antibody

Journal of Clinical Microbiology
J FedorkoR H Parker

Abstract

Modifications of the staphylococcal counterimmunoelectrophoresis technique were evaluated to determine how variations in the procedure affected results. Neither a buffer pH range of 7.8 to 9.0 nor buffer molarity of 0.015 or 0.025 when tested in combinations caused appreciable differences. However, use of different agar preparations or delay in addition of antigen to the test slide altered the location of the precipitin band. Agarose was found to be more sensitive in determining the serum precipitin titer and provided a better photographic record than either Ionagar or Noble agar.

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Mentioned in this Paper

Immunoelectrophoresis
Agar
Precipitins
Genus staphylococcus
Staphylococcal Infections
Differential Diagnosis
Antigens
Countercurrent Electrophoresis Measurement
Sepharose
Staphylococcus aureus

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