PMID: 6167505Jan 1, 1981Paper

Counterstain-enhanced chromosome banding

Human Genetics
D Schweizer

Abstract

Chromosome staining, in which at least one member of a pair or triplet of DNA binding dyes is fluorescent whereas the others act as counterstain, is reviewed. Appropriately chosen combinations of fluorescent dyes and counterstains can be employed to enhance general chromosome banding patterns, or to induce specific regional banding patterns. Some pairs of dyes which exhibit complementary DNA binding specificity, A-T/G-C or G-C/A-T, provide enhanced definition of positive or reverse banding patterns. Dye combinations of the type A-T/A-T, that include two DNA stains with similar specificity but non-identical binding modes, produce a specific pattern of brightly fluorescent heterochromatic regions (DA-DAPI bands). In man, the method highlights the C bands of chromosomes 1, 9, 15, 16, and the Y. Certain dye triplets of the type G-C/A-T/A-T, which include two spectroscopically separated fluorescent stains with reciprocal DNA base pair binding specificities and a non-fluorescent A-T binding counterstain, can be used to highlight selectively, in the appropriate wavelength ranges, either R bands or DA-DAPI bands. Applications of these techniques in human cytogenetics are described. The potential of the new methodology for detecting and...Continue Reading

Citations

Jun 1, 1994·The Histochemical Journal·A R LeitchI J Leitch
Oct 1, 1992·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·M FlajšhansS Dobosz
Jul 1, 1988·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·B MayrP Rab
Jan 1, 1993·Mammalian Genome : Official Journal of the International Mammalian Genome Society·U M Sarmiento, K Kadavil
Jan 1, 1993·Mammalian Genome : Official Journal of the International Mammalian Genome Society·U M SarmientoS S Rishi
Jan 1, 1993·Mammalian Genome : Official Journal of the International Mammalian Genome Society·U M SarmientoS S Rishi
Jan 1, 1993·Mammalian Genome : Official Journal of the International Mammalian Genome Society·U M Sarmiento, K Kadavil
Oct 1, 1994·Mammalian Genome : Official Journal of the International Mammalian Genome Society·M StevanovicP Goodfellow
May 1, 1994·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·D G Bedo
May 1, 1994·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·M T LoonesP Vigny
Mar 1, 1994·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·M D López-LeónJ P Camacho
Apr 1, 1990·Chromosoma·H Zacharias
Jun 1, 1989·Journal of Molecular Evolution·G P Holmquist
Mar 3, 2010·Mammalian Genome : Official Journal of the International Mammalian Genome Society·David J Bernard, Robert L Nussbaum
Apr 5, 2007·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Agata KowalskaPeter F Ambros
May 1, 1982·Cancer Genetics and Cytogenetics·A T Sumner
Mar 15, 1985·Cancer Genetics and Cytogenetics·Z Almássy, M Holmberg
Feb 1, 1987·Cancer Genetics and Cytogenetics·M TaniwakiT Takino
Oct 1, 1988·Cancer Genetics and Cytogenetics·T Schwarzacher-RobinsonL S Cram
Dec 1, 1990·Cancer Genetics and Cytogenetics·H HuberD Schweizer
Dec 10, 2003·Genome Génome / Conseil National De Recherches Canada·Trude Schwarzacher
Jun 1, 1996·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·A de CapoaA Niveleau
Jan 1, 1988·Chromosoma·M Schmid, C G de Almeida
Nov 1, 1991·The Histochemical Journal·J C StockertP Del Castillo
Jan 1, 1984·Chromosoma·M KaelblingO J Miller
Jan 1, 1987·Chromosoma·R K MoyzisT Schwarzacher-Robinson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.