PMID: 641379May 1, 1978Paper

Counting and sizing of epidermal cells in normal human skin

The Journal of Investigative Dermatology
P R BergstresserJ R Taylor

Abstract

During keratinocyte maturation, individual cells undergo an orderly succession of biochemical and structural changes. In certain skin disorders alterations in keratinocyte numbers, volumes, and epidermal skin thickness occur. To assess such alterations and to provide base line values for normal human epidermis, a computer assisted histologic technique was developed. Skin biopsies were taken from normal skin on the forearm, back and thigh of 6 adult men. Whole specimens of epidermis were separated from the dermis with collagenase, fixed, stained, and mounted for microscopic examination. From the three dimensional coordinates of epidermal nuclei, epidermal cell volumes, surface density of epidermal cells, and epidermal thickness were determined. Measurement of cell volumes in this way compared favorably with electronic cell sizing of disaggregated epidermal cells in matched samples. The mean densities of nucleated cells per 10(4) mu2 surface area were 452, 483, and 487 for the forearm, back and thigh respectively. This technique will be used to make similar assessments in disorders of abnormal keratinocyte maturation.

Citations

Jan 1, 1985·Clinical and Experimental Dermatology·S M MorleyI Sarkany
Jul 18, 2002·The Australasian Journal of Dermatology·Johanna KuchelRoss Barnetson
Jan 9, 2015·Journal of the Royal Society, Interface·Hong ZhangJin Yang
Jan 1, 1985·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·J K LarsenI J Christensen
Mar 8, 2018·Physiological Measurement·David A NelsonJames M Downey
Jan 1, 1981·Archives of Dermatological Research·P M Elias
Jan 1, 1981·International Journal of Dermatology·P M Elias
May 1, 1985·Journal of Oral Pathology·E E AhlforsP R Bergstresser
Jan 1, 1981·Cell and Tissue Research·W Müller-Glauser
Mar 1, 1987·Cell and Tissue Kinetics·G M Morris, J W Hopewell
Jun 1, 1980·The British Journal of Dermatology·P R Bergstresser, S L Chapman
Oct 23, 2020·Cancer Discovery·Joanna C FowlerPhilip H Jones
Apr 3, 2021·Experimental Dermatology·Christoph H TrippPatrizia Stoitzner
Nov 26, 1999·The Journal of Investigative Dermatology·Y OdaT Mauro
Nov 1, 1984·The Journal of Investigative Dermatology·A Takashima, F Grinnell
Oct 28, 2005·American Journal of Human Genetics·Anna M G PasmooijMarcel F Jonkman

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The Journal of Investigative Dermatology
R D Sontheimer, P R Bergstresser
The Journal of Investigative Dermatology
R A Briggaman
Advances in Biochemical Engineering/biotechnology
Charles E Butler, Dennis P Orgill
Surgical and Radiologic Anatomy : SRA
Y Lee, K Hwang
© 2021 Meta ULC. All rights reserved