Counting charges on membrane-bound peptides

Chemical Science
Alicia C McGeachyFranz M Geiger

Abstract

Quantifying the number of charges on peptides bound to interfaces requires reliable estimates of (i) surface coverage and (ii) surface charge, both of which are notoriously difficult parameters to obtain, especially at solid/water interfaces. Here, we report the thermodynamics and electrostatics governing the interactions of l-lysine and l-arginine octamers (Lys8 and Arg8) with supported lipid bilayers prepared from a 9 : 1 mixture of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and 1,2-dimyristoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (sodium salt) (DMPG) from second harmonic generation (SHG) spectroscopy, quartz crystal microbalance with dissipation monitoring (QCM-D) and nanoplasmonic sensing (NPS) mass measurements, and atomistic simulations. The combined SHG/QCM-D/NPS approach provides interfacial charge density estimates from mean field theory for the attached peptides that are smaller by a factor of approximately two (0.12 ± 0.03 C m-2 for Lys8 and 0.10 ± 0.02 C m-2 for Arg8) relative to poly-l-lysine and poly-l-arginine. These results, along with atomistic simulations, indicate that the surface charge density of the supported lipid bilayer is neutralized by the attached cationic peptides. Moreover, the number of ...Continue Reading

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Citations

Feb 10, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Hui YinJoel A Pedersen
Feb 26, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Harnoor Singh SacharSiddhartha Das
Jan 1, 2020·ACS Applied Materials & Interfaces·Naomi DalchandFranz M Geiger
Jul 3, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Cai YaoQi Wang
Jul 2, 2019·The Journal of Physical Chemistry. B·Mavis D BoamahFranz M Geiger
Nov 3, 2021·Physical Chemistry Chemical Physics : PCCP·Guillaume Le BretonClaire Loison

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Methods Mentioned

BETA
fluorescence assay
surface plasmon resonance
QCM-D

Software Mentioned

QTools
OpenMM
CHARMM36
NAMD
- GUI
QCM
CHARMM
GUI

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