Counting total cell numbers microscopically on stained films

Letters in Applied Microbiology
C JiangT T Li

Abstract

Two main sources of error in the conventional stained film cell count method, i.e. errors from imperfect spreading of the suspension and uneven distribution of cells in the film, have been eliminated by counting all the cells in the film. Staining solution and sodium alginate were added to the suspension before spreading over the slide. With a microsyringe, 20-microliters suspension was spread in two bands of ca 1.7 mm x 60 mm each. Then total cells could be counted easily in the x 100 magnification field by moving the slide in one direction. Results were satisfactory. Coefficients of variation were 2.6 and 1.1% in two separate yeast determinations each with five replicates. For counting higher magnifications, a suitable image analyser for automating the method is believed worthy of study.

References

Dec 1, 1985·The Journal of Applied Bacteriology·U M Rodrigues, R G Kroll
Jan 1, 1989·Journal of Microbiological Methods·A SinghG A McFeters
Mar 1, 1940·Journal of Bacteriology·J H Hanks, D F James
Aug 20, 1988·Biotechnology and Bioengineering·H L Adams, C R Thomas

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Citations

Jul 1, 1996·Letters in Applied Microbiology·C JiangY Ma

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