Country-Wide Surveillance of Molecular Markers of Antimalarial Drug Resistance in Senegal by Use of Positive Malaria Rapid Diagnostic Tests

The American Journal of Tropical Medicine and Hygiene
Magatte NdiayeMichael Alifrangis

Abstract

In Senegal, antimalarial drugs used in treatment and prevention of malaria are one of the main reasons for the current success in controlling malaria. However, the successful control of malaria is highly dependent on continued effectiveness of these drugs which may be compromised by the spread of drug resistance. Therefore, surveillance of drug resistance in the malaria parasites is essential. The objective of this pilot study was to test the feasibility of routinely sampled malaria rapid diagnostic tests (RDTs) at a national scale to assess the temporal changes in the molecular profiles of antimalarial drug resistance markers of Plasmodium falciparum parasites. Overall, 9,549 positive malaria RDTs were collected from 14 health facilities across the country. A limited random set of RDTs were analyzed regarding Pfcrt gene polymorphisms at codon 72-76. Overall, a high but varied prevalence (> 50%) of the wild-type CVMNK haplotype was observed including a higher CVMNK prevalence in the northern part (75%) compared with the southern part of the country (59%). With caution, the study provides a proof of concept that reuse of discarded P. falciparum positive RDTs can be applied in large-scale surveillance of antimalarial drug resista...Continue Reading

References

Mar 16, 2000·Transactions of the Royal Society of Tropical Medicine and Hygiene·P BrasseurPiero Olliaro
Aug 1, 1993·Parasitology Today·J WoodenC H Sibley
Sep 14, 2013·Trends in Parasitology·Inbarani Naidoo, Cally Roper
Dec 20, 2013·Nature·Frederic ArieyDidier Ménard
Jul 25, 2014·The Lancet Infectious Diseases·Cally RoperPascal Ringwald

Methods Mentioned

BETA
genotyping
PCR
PCRs
PCR-SSOP-ELISA

Related Concepts

Related Feeds

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.