Coupled electrophysiological recording and single cell transcriptome analyses revealed molecular mechanisms underlying neuronal maturation

Protein & Cell
Xiaoying ChenYi E Sun

Abstract

The mammalian brain is heterogeneous, containing billions of neurons and trillions of synapses forming various neural circuitries, through which sense, movement, thought, and emotion arise. The cellular heterogeneity of the brain has made it difficult to study the molecular logic of neural circuitry wiring, pruning, activation, and plasticity, until recently, transcriptome analyses with single cell resolution makes decoding of gene regulatory networks underlying aforementioned circuitry properties possible. Here we report success in performing both electrophysiological and whole-genome transcriptome analyses on single human neurons in culture. Using Weighted Gene Coexpression Network Analyses (WGCNA), we identified gene clusters highly correlated with neuronal maturation judged by electrophysiological characteristics. A tight link between neuronal maturation and genes involved in ubiquitination and mitochondrial function was revealed. Moreover, we identified a list of candidate genes, which could potentially serve as biomarkers for neuronal maturation. Coupled electrophysiological recording and single cell transcriptome analysis will serve as powerful tools in the future to unveil molecular logics for neural circuitry functions.

References

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Citations

Oct 24, 2018·Frontiers in Molecular Neuroscience·Shreejoy J TripathyPaul Pavlidis
Apr 25, 2018·Frontiers in Neuroscience·Godwin SokporTran Tuoc
Jan 17, 2020·International Journal of Molecular Sciences·Bruno SterliniAnna Corradi
Jan 30, 2021·Frontiers in Neuroscience·Austin P Passaro, Steven L Stice
Aug 4, 2021·Human Genetics·Daniel R RomanoRick F Nelson

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Datasets Mentioned

BETA
GSE
77564

Methods Mentioned

BETA
dissection
PCA
dissect
PCR

Software Mentioned

WGCNA
VisANT
Neurolucida
mini Analysis Program
pCLAMP10
Clampfit
Cufflinks

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