PMID: 37342May 7, 1979

Coupled transport of protons and anions through lipid bilayer membranes containing a long-chain secondary amine

The Journal of Membrane Biology
J Gutknecht, A Walter

Abstract

Transport of protons and halide ions through planar lipid bilayers made from egg lecithin and a long-chain secondary amine (n-lauryl [trialkylmethyl] amine) in n-decane was studied. Net proton fluxes were measured with a pH electrode, and halide fluxes were measured with 82Br- and 36Cl-. In membranes containing the secondary amine, a large net proton flux was produced either by a Br- gradient with symmetrical pH or by a pH gradient with symmetrical Br-, but not by a pH gradient in Br--free solutions. This H+ flux was electrically silent (nonconductive), and the H+ permeability coefficient was greater than 10(-3) cm sec-1 in 0.1 M NaBr. In Br--free solutions, H+ selectivity was observed electrically by measuring conductances and zero-current potentials generated by H+ activity gradients. The permeability coefficient for this ionic (conductive) H+ flux was about 10(-5) cm sec-1, several orders of magnitude smaller than the H+ permeability of the electroneutral pathway. Large electroneutral Br- exchange fluxes occurred under symmetrical conditions, and the permeability coefficient for Br- exchange was about 10(-3) cm sec-1 at pH 5. The one-way Br- flux was inhibited by substituting SO4= for Br- on the "trans" side of the membrane....Continue Reading

References

Aug 26, 1976·The Journal of Membrane Biology·M L Jennings
Nov 1, 1976·The Journal of Physiology·J Funder, J O Wieth
Mar 1, 1977·The Journal of General Physiology·P A KnaufA Rothstein
Jan 1, 1977·Physiological Reviews·E M Wright, J M Diamond
Oct 6, 1972·Science·P Läuger
Jul 14, 1966·Annals of the New York Academy of Sciences·G M Shean, K Sollner
Jul 1, 1967·The Journal of General Physiology·T E AndreoliD C Tosteson
Sep 6, 1958·Nature·A D BANGHAMG V SEAMAN

Citations

Aug 21, 1980·The Journal of Membrane Biology·J Gutknecht, A Walter
Jun 1, 2010·Biochimica Et Biophysica Acta·Tatyana I RokitskayaVladimir P Skulachev
Jan 20, 2015·Analytical Chemistry·Edmund C M TseAndrew A Gewirth
Nov 18, 2004·Journal of Lipid Research·Kellen BrunaldiJoaquim Procopio
Jun 15, 2011·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Rajendra BadweIndraneel Mittra

Related Concepts

Amines
Bromides
Chloride Ion Level
Electric Conductivity
Hydrogen
Hydrogen-Ion Concentration
Resting Potentials
Membranes, Artificial
Permeability

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