Coupling between [arginine8]-vasopressin-activated increases in protein tyrosine phosphorylation and cellular calcium in A7r5 aortic smooth muscle cells

Archives of Biochemistry and Biophysics
N Kaplan, J Di Salvo

Abstract

Effects of genistein, a tyrosine kinase inhibitor, on increases in [Ca2+]i and protein tyrosine phosphorylation induced by 20 nM [arginine 8]vasopressin (AVP) were studied in A7r5 aortic smooth muscle cells. In fura-2-loaded cells, AVP induced a rapid (0.5-2 min) transient increase in [Ca2+]i that was followed by a smaller sustained increase in [Ca2+]i. In 66% of the cells, the transient response involved both influx of extracellular Ca2+ and release of intracellular Ca2+: influx accounted for 6% of the response, and release accounted for 40%. However, in 34% of the cells, the relative contribution of influx and release during the transient could not be assessed. In all cells, the smaller sustained response was entirely dependent on extracellular Ca2+. Genistein (148 microM) always blocked the transient and sustained components of the Ca2+ response showing that both influx and release were genistein-sensitive. Isobestic fluorescence analysis, in medium containing 0.5 mM Mn2+ in place of Ca2+, showed that the influx pathway was selective because it did not conduct Mn2+. It also confirmed that Ca2+ release was blocked by genistein. In contrast, 105 microM lavendustin A, a different tyrosine kinase inhibitor, suppressed the transi...Continue Reading

Citations

May 22, 2002·European Journal of Pharmacology·Timothy V MurphyMichael A Hill
Oct 22, 2003·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Alexander Y. ZubkovJohn H. Zhang
Mar 8, 2000·Biochemical Pharmacology·S AlcónM J Pozo
Sep 1, 1997·Archives of Biochemistry and Biophysics·S R NelsonJ Di Salvo
Oct 12, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Derek S DamronPaul A Murray
May 1, 1997·The American Journal of Physiology·J R SchellingG R Dubyak
Jun 3, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·B TolloczkoJ G Martin
Nov 11, 1998·Archives of Biochemistry and Biophysics·N Kaplan-Albuquerque, J Di Salvo

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