Coupling between pore formation and phase separation in charged lipid membranes

Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics
Hiroki HimenoMasahiro Takagi

Abstract

We investigated the effect of charge on the membrane morphology of giant unilamellar vesicles (GUVs) composed of various mixtures containing charged lipids. We observed the membrane morphologies by fluorescent and confocal laser microscopy in lipid mixtures consisting of a neutral unsaturated lipid [dioleoylphosphatidylcholine (DOPC)], a neutral saturated lipid [dipalmitoylphosphatidylcholine (DPPC)], a charged unsaturated lipid [dioleoylphosphatidylglycerol (DOPG((-)))], a charged saturated lipid [dipalmitoylphosphatidylglycerol (DPPG((-)))], and cholesterol (Chol). In binary mixtures of neutral DOPC-DPPC and charged DOPC-DPPG((-))), spherical vesicles were formed. On the other hand, pore formation was often observed with GUVs consisting of DOPG((-))) and DPPC. In a DPPC-DPPG((-)))-Chol ternary mixture, pore-formed vesicles were also frequently observed. The percentage of pore-formed vesicles increased with the DPPG((-))) concentration. Moreover, when the head group charges of charged lipids were screened by the addition of salt, pore-formed vesicles were suppressed in both the binary and ternary charged lipid mixtures. We discuss the mechanisms of pore formation in charged lipid mixtures and the relationship between phase sep...Continue Reading

References

Nov 27, 1987·Biochimica Et Biophysica Acta·J H IpsenM J Zuckermann
Nov 1, 1973·Zeitschrift Für Naturforschung. Teil C: Biochemie, Biophysik, Biologie, Virologie·W Helfrich
Apr 1, 1981·Biophysical Journal·E BoroskeW Helfrich
Apr 8, 1993·Biochimica Et Biophysica Acta·D V Zhelev, D Needham
Jun 5, 1997·Nature·K Simons, E Ikonen
Jun 20, 2001·Nature Reviews. Molecular Cell Biology·K Simons, D Toomre
Dec 18, 2002·Physical Review Letters·Sarah L Veatch, Sarah L Keller
Mar 15, 2005·Journal of Cell Science·Lawrence Rajendran, Kai Simons
Aug 11, 2005·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Ira R CookeMarkus Deserno
Dec 2, 2005·Nature·Harvey T McMahon, Jennifer L Gallop
Feb 9, 2006·PLoS Biology·Gabor Juhasz, Thomas P Neufeld
Nov 8, 2006·The European Physical Journal. E, Soft Matter·M Kaga, T Ohta
Nov 14, 2006·PLoS Computational Biology·Kerwyn Casey HuangNed S Wingreen
Feb 24, 2007·Nature Reviews. Molecular Cell Biology·Robert G Parton, Kai Simons
Jul 27, 2007·Current Opinion in Cell Biology·Volker Haucke, Gilbert Di Paolo
Aug 28, 2007·The Journal of Physical Chemistry. B·Tsutomu HamadaMasahiro Takagi
Dec 14, 2007·Journal of Lipid Research·Michael Schlame
Apr 18, 2008·The European Physical Journal. E, Soft Matter·Y SakumaS Komura
Jun 10, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Karin A RiskeM Teresa Lamy
Jan 2, 2010·Science·Daniel Lingwood, Kai Simons
Jan 5, 2010·Biochimica Et Biophysica Acta·Cíntia C Vequi-SuplicyRumiana Dimova
Apr 7, 2010·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Tsutomu HamadaMasahiro Takagi
Jul 21, 2010·Biophysical Journal·Yuka SakumaMasayuki Imai
Jul 29, 2010·Journal of the American Chemical Society·Tsutomu HamadaMasahiro Takagi
Mar 11, 2011·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Young Zoon YoonPietro Cicuta
Apr 21, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Paul A BealesT Kyle Vanderlick
Jun 2, 2011·Cold Spring Harbor Perspectives in Biology·Kai Simons, Julio L Sampaio
Nov 9, 2011·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Naofumi ShimokawaDavid Andelman
Mar 30, 2012·Biophysical Journal·Mingyang HuMarkus Deserno
Jan 12, 2013·Current Biology : CB·Claudia Dall'ArmiGilbert Di Paolo
Apr 12, 2013·Proceedings of the National Academy of Sciences of the United States of America·Chris L BergstromJohn T Groves
Jun 26, 2013·Biophysical Journal·Matthew C BlosserSarah L Keller
May 2, 2014·Biochimica Et Biophysica Acta·Salome PataraiaRumiana Dimova
Aug 15, 2014·The European Physical Journal. E, Soft Matter·Yotam Y AvitalOded Farago
Aug 27, 2014·Soft Matter·Hiroki HimenoMasahiro Takagi

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Citations

May 5, 2016·The Journal of Physical Chemistry. B·Naofumi ShimokawaDavid Andelman
Sep 8, 2019·The Journal of Chemical Physics·Hiroshi Noguchi
Aug 29, 2019·ACS Omega·Candelaria I CámaraNatalia Wilke
Dec 24, 2021·Journal of Chemical Theory and Computation·Klemen BohincSimone Spada

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