Coupling between replication and packaging of flavivirus RNA: evidence derived from the use of DNA-based full-length cDNA clones of Kunjin virus

Journal of Virology
A A KhromykhE G Westaway

Abstract

In order to study whether flavivirus RNA packaging is dependent on RNA replication, we generated two DNA-based Kunjin virus constructs, pKUN1 and pKUN1dGDD, allowing continuous production of replicating (wild-type) and nonreplicating (with a deletion of the NS5 gene RNA-polymerase motif GDD) full-length Kunjin virus RNAs, respectively, via nuclear transcription by cellular RNA polymerase II. As expected, transfection of pKUN1 plasmid DNA into BHK cells resulted in the recovery of secreted infectious Kunjin virions. Transfection of pKUN1dGDD DNA into BHK cells, however, did not result in the recovery of any secreted virus particles containing encapsidated dGDD RNA, despite an apparent accumulation of this RNA in cells demonstrated by Northern blot analysis and its efficient translation demonstrated by detection of correctly processed labeled structural proteins (at least prM and E) both in cells and in the culture fluid using coimmunoprecipitation analysis with anti-E antibodies. In contrast, when dGDD RNA was produced even in much smaller amounts in pKUN1dGDD DNA-transfected repBHK cells (where it was replicated via complementation), it was packaged into secreted virus particles. Thus, packaging of defective Kunjin virus RNA co...Continue Reading

References

Jan 1, 1987·Advances in Virus Research·E G Westaway
Feb 1, 1997·Journal of Virology·A A Khromykh, E G Westaway
Nov 26, 1997·Journal of Virology·B D Lindenbach, C M Rice
Dec 16, 1998·Journal of Virology·C I NugentK Kirkegaard
Mar 9, 2000·Journal of Virology·A A KhromykhE G Westaway

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Citations

Jul 1, 2008·Nature Reviews. Microbiology·Catherine L MurrayCharles M Rice
Jan 20, 2005·The Journal of Experimental Medicine·Deng TaoRuth S Nussenzweig
Jul 8, 2010·Antimicrobial Agents and Chemotherapy·Min QingPei-Yong Shi
Mar 14, 2008·Journal of Virology·Jason Y LeungAlexander A Khromykh
Apr 20, 2007·Journal of Virology·Miguel A SanzLuis Carrasco
Mar 22, 2002·Journal of Virology·Itaru AnrakuAlexander A Khromykh
Sep 6, 2005·Journal of Virology·Alec J HirschJay A Nelson
Feb 17, 2006·Annual Review of Phytopathology·A L N Rao
May 12, 2011·PloS One·Dongsheng LiJohn Aaskov
Oct 24, 2009·PLoS Pathogens·Marcelo M SamsaAndrea V Gamarnik
May 3, 2011·PLoS Pathogens·Miriam TriyatniBertrand Saunier
Feb 19, 2010·Future Medicinal Chemistry·Brian J GeissSusan M Keenan
Jan 1, 2013·Uirusu·Ryosuke Suzuki, Eiji Konishi
Aug 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Roy A HallAlexander A Khromykh
Nov 26, 2003·Journal of Evolutionary Biology·D C Krakauer, N L Komarova
Jun 7, 2014·Annual Review of Phytopathology·Pooja Saxena, George P Lomonossoff
Oct 5, 2010·Journal of Theoretical Biology·Alison K McLeanMark M Tanaka
Apr 26, 2008·Virus Research·Ricardo L A SilvaGene H MacDonald
Feb 5, 2008·Journal of Virological Methods·Junko MaedaAkihiko Maeda
Sep 30, 2005·Traffic·Jason Mackenzie
Sep 9, 2005·Virus Research·Xiao Tong, Bruce A Malcolm

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