Coupling confocal fluorescence detection and recirculating microfluidic control for single particle analysis in discrete nanoliter volumes

Lab on a Chip
C M PuleoTza-Huei Wang

Abstract

The recent proliferation of platforms designed to handle arrays of nano- and picolitre volumes is in response to the need to perform biological assays on discrete entities, such as single cells. However, a critical challenge associated with this trend for in vitro compartmentalization is the need for highly sensitive, yet low-volume detection platforms. In this paper, we coupled confocal fluorescence detection with recirculating microfluidic control to perform single particle DNA assays within five nL chambers. The performance of this low-volume assay was shown to match that of traditional single molecule detection platforms. However, volume requirements per measurement were nearly 3 orders of magnitude less than conventional systems, enabling future integration with lab-on-a-chip systems that require discrete or digitalized sample processing.

References

Mar 10, 2004·Lab on a Chip·Phil PaikRichard B Fair
Mar 17, 2004·Nature Biotechnology·Jong Wook HongStephen R Quake
Apr 27, 2004·Lab on a Chip·Andrew J de Mello, Nigel Beard
Apr 14, 2005·Journal of the American Chemical Society·Tza-Huei WangChih-Ming Ho
Dec 22, 2005·Nature Methods·Lori A NeelyJenny Rooke
Dec 28, 2005·Nature Materials·Chun-Yang ZhangTza-Huei Wang
Apr 29, 2006·Analytical Chemistry·Joshua S MarcusStephen R Quake
Aug 2, 2006·Analytical Chemistry·Chun-Yang Zhang, Lawrence W Johnson
Nov 14, 2006·Proceedings of the National Academy of Sciences of the United States of America·Luigi WarrenStephen R Quake
Oct 13, 2007·Analytical Chemistry·N Reginald BeerBill W Colston

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Citations

Apr 8, 2009·Lab on a Chip·Christopher M Puleo, Tza-Huei Wang
Feb 2, 2011·Chemical Communications : Chem Comm·Rohan T Ranasinghe, Tom Brown
Jul 31, 2012·Lab on a Chip·Tushar D RaneTza-Huei Wang
Dec 14, 2011·Sensors·Charles H VannoyUlrich J Krull
May 19, 2010·Lab on a Chip·Smitha S VargheseStephen C Trowell
Jan 29, 2016·Lab on a Chip·Sarah M FriedrichTza-Huei Wang
Jan 13, 2010·Lab on a Chip·T D RaneT H Wang
Apr 13, 2017·Lab on a Chip·Zachary EstlackJungkyu Kim
Sep 14, 2018·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jerome CarpenterRichard Superfine
Jul 17, 2021·Applied Spectroscopy·Nathan A GiauqueSteven R Goates
Jan 1, 2016·ACS Sensors·Brandon RoarkKirill A Afonin

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