Coupling desorption electrospray ionization with ion mobility/mass spectrometry for analysis of protein structure: evidence for desorption of folded and denatured States

The Journal of Physical Chemistry. B
Sunnie MyungDavid E Clemmer

Abstract

A desorption electrospray ionization (DESI) source has been coupled to an ion mobility time-of-flight mass spectrometer for the analysis of proteins. Analysis of solid-phase horse heart cytochrome c and chicken egg white lysozyme proteins with different DESI solvents and conditions shows similar mass spectra and charge state distributions to those formed when using electrospray to analyze these proteins in solution. The ion mobility data show evidence for compact ion structures [when the surface is exposed to a spray that favors retention of "nativelike" structures (50:50 water:methanol)] or elongated structures [when the surface is exposed to a spray that favors "denatured" structures (49:49:2 water:methanol:acetic acid)]. The results suggest that the DESI experiment is somewhat gentler than ESI and under appropriate conditions, it is possible to preserve structural information throughout the DESI process. Mechanisms that are consistent with these results are discussed.

References

Mar 1, 1991·Rapid Communications in Mass Spectrometry : RCM·J A LooR D Smith
Jul 20, 1990·Journal of Molecular Biology·G W BushnellG D Brayer
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·T D WoodF W McLafferty
Apr 2, 1996·Proceedings of the National Academy of Sciences of the United States of America·D S GrossE R Williams
Sep 1, 1995·Analytical Chemistry·A HirabayashiH Koizumi
Jun 13, 1998·Analytical Chemistry·C S HoaglundD E Clemmer
Oct 10, 1994·Physical Review Letters·J M HunterJ E Bower
Jun 14, 2000·Analytical Chemistry·T KimR D Smith
May 30, 2002·Journal of the American Chemical Society·Kathrin BreukerFred W McLafferty
Jan 10, 2003·Nature·Denis DuftThomas Leisner
Mar 28, 2003·Analytical Chemistry·Zoltan TakatsKaroly Vekey
Jun 10, 2003·Journal of Mass Spectrometry : JMS·Kentaro Yamaguchi
Sep 11, 2004·Molecular & Cellular Proteomics : MCP·Dmitry S GembitskyPaul Tempst
Aug 1, 1993·Journal of the American Society for Mass Spectrometry·T Covey, D J Douglas

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Citations

Feb 12, 2008·Analytical and Bioanalytical Chemistry·Sofie P PasilisSusanne Schorcht
Feb 28, 2009·Analytical and Bioanalytical Chemistry·Larissa S FennJohn A McLean
Jun 4, 2010·Analytical and Bioanalytical Chemistry·Rosana M AlbericiMarcos N Eberlin
Apr 8, 2011·Journal of the American Society for Mass Spectrometry·Chang-Ching ChanAthula B Attygalle
Aug 22, 2012·Journal of the American Society for Mass Spectrometry·Kevin A DouglassAndre R Venter
Jun 8, 2007·Journal of the American Society for Mass Spectrometry·Xiaoyun LiuDavid E Clemmer
Jan 12, 2008·Journal of the American Society for Mass Spectrometry·Marcela NefliuR Graham Cooks
Oct 28, 2008·Journal of the American Society for Mass Spectrometry·Zhixin Miao, Hao Chen
Jun 15, 2010·Journal of the American Society for Mass Spectrometry·Chang-Ching ChanAthula B Attygalle
Jul 14, 2010·Journal of the American Society for Mass Spectrometry·Zhixin MiaoHao Chen
Apr 29, 2010·Chemical Reviews·Kamila Chughtai, Ron M A Heeren
Mar 8, 2008·Nature Protocols·Justin M WisemanR Graham Cooks
Apr 7, 2010·The Analyst·Roberto Fernández-MaestreHerbert Henderson Hill
Jul 19, 2008·Annual Review of Analytical Chemistry·Brian C BohrerDavid E Clemmer
May 23, 2012·Bioanalysis·Christopher Singleton
Jun 7, 2014·Journal of the American Society for Mass Spectrometry·Joel D KeelorFacundo M Fernández
Jan 22, 2013·Annual Review of Physical Chemistry·Abraham K Badu-TawiahR Graham Cooks
May 13, 2008·Journal of Mass Spectrometry : JMS·Chris Petucci, Jason Diffendal
Jun 20, 2008·Journal of Mass Spectrometry : JMS·Sofie P PasilisSusanne Schorcht
Sep 22, 2010·Journal of Mass Spectrometry : JMS·Abdil Özdemir, Chung-Hsuan Chen
May 16, 2013·Journal of Mass Spectrometry : JMS·Kevin Aart Douglass, Andre R Venter
Sep 8, 2011·Mass Spectrometry Reviews·Zhong-Ping Yao
Oct 4, 2014·Rapid Communications in Mass Spectrometry : RCM·Riikka-Marjaana RäsänenTiina J Kauppila
Dec 18, 2013·Mass Spectrometry Reviews·Xuelu Ding, Yixiang Duan
Jun 14, 2015·Annual Review of Analytical Chemistry·William D Hoffmann, Glen P Jackson
Mar 18, 2017·Critical Reviews in Analytical Chemistry·Hubert BylińskiJacek Namieśnik
Jan 13, 2009·European Journal of Mass Spectrometry·Jaroslav PólVladimír Havlícek
Apr 3, 2018·Journal of Mass Spectrometry : JMS·Klaudia I KocurekHelen J Cooper
Oct 20, 2006·Rapid Communications in Mass Spectrometry : RCM·Qizhi HuR Graham Cooks
Jan 18, 2008·Journal of Mass Spectrometry : JMS·Abu B KanuHerbert H Hill
Mar 6, 2008·Rapid Communications in Mass Spectrometry : RCM·Tiina J KauppilaRisto Kostiainen
Nov 21, 2008·Rapid Communications in Mass Spectrometry : RCM·Joanna E BarbaraDavid H Powell
Jun 29, 2010·Rapid Communications in Mass Spectrometry : RCM·Stefanie Gerbig, Zoltán Takáts
Sep 22, 2010·Rapid Communications in Mass Spectrometry : RCM·Chang-Ching ChanAthula B Attygalle
Feb 4, 2011·Rapid Communications in Mass Spectrometry : RCM·Daiki Asakawa, Kenzo Hiraoka
Feb 24, 2007·Rapid Communications in Mass Spectrometry : RCM·Gushinder Kaur-AtwalColin S Creaser
Feb 20, 2021·Journal of the American Society for Mass Spectrometry·Bin Yan, Josephine Bunch

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