Coupling Drosophila melanogaster Cryptochrome Light Activation and Oxidation of the Kvβ Subunit Hyperkinetic NADPH Cofactor

The Journal of Physical Chemistry. B
Gongyi HongThorsten Ritz

Abstract

Motivated by the observations on the involvement of light-induced processes in the Drosophila melanogaster cryptochrome (DmCry) in regulation of the neuronal firing rate, which is achieved by a redox-state change of its voltage-dependent K+ channel Kvβ subunit hyperkinetic (Hk) reduced nicotinamide adenine dinucleotide phosphate (NADPH) cofactor, we propose in this work two hypothetical pathways that may potentially enable such coupling. In the first pathway, triggered by blue-light-induced formation of a radical pair [FAD•-TRP•+] in DmCry, the hole (TRP•+) may hop to Hk, for example, through a tryptophan chain and oxidize NADPH, possibly leading to inhibition of the N-terminus inactivation in the K+ channel. In a second possible pathway, DmCry's FAD•- is reoxidized by molecular oxygen, producing H2O2, which then diffuses to Hk and oxidizes NADPH. In this work, by applying a combination of quantum and empirical-based methods for free-energy calculations, we find that the oxidation of NADPH by TRP•+ or H2O2 and the reoxidation of FAD•- by O2 are thermodynamically feasible. Our results may have an implication in identifying a magnetic sensing signal transduction pathway, specifically upon Drosophila's Hk NADPH cofactor oxidation,...Continue Reading

References

Jul 1, 1988·The Biochemical Journal·P M Wood
Jan 1, 1986·Annals of the New York Academy of Sciences·D A Case, J A McCammon
Jun 15, 1983·Biochemical and Biophysical Research Communications·R S Bodaness
Apr 2, 1980·Biochimica Et Biophysica Acta·R F Anderson
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·S W ChouinardB Ganetzky
Feb 2, 2000·Biophysical Journal·T RitzK Schulten
Jun 5, 2003·Proteins·Rong ChenZhiping Weng
Mar 12, 2004·Annual Review of Physical Chemistry·M W Schmidt, M S Gordon
Apr 26, 2005·Nucleic Acids Research·Yang Zhang, Jeffrey Skolnick
Jul 9, 2005·Science·Stephen B LongRoderick Mackinnon
Nov 23, 2005·Bioinformatics·Konstantin ArnoldTorsten Schwede
Sep 13, 2007·Nature Reviews. Molecular Cell Biology·Benoît D'Autréaux, Michel B Toledano
Jan 29, 2008·The Journal of Biological Chemistry·Yaping PanMing Zhou
Jul 22, 2008·Nature·Robert J GegearSteven M Reppert
Sep 23, 2008·Nature Chemical Biology·Yaping PanMing Zhou
Jan 9, 2009·Proceedings of the National Academy of Sciences of the United States of America·Christopher T Rodgers, P J Hore
Apr 10, 2009·PLoS Biology·Taishi YoshiiCharlotte Helfrich-Förster
Apr 23, 2009·Biophysical Journal·Thorsten RitzWolfgang Wiltschko
Jun 17, 2009·Biophysical Journal·Ilia A Solov'yov, Klaus Schulten
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Xiaorui ChenJianpeng Ma
Dec 29, 2010·Proceedings of the National Academy of Sciences of the United States of America·Nuri OzturkAziz Sancar
Mar 10, 2011·Science·Keri J FogleTodd C Holmes
Mar 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·Yaping PanMing Zhou
Apr 30, 2011·Annual Review of Plant Biology·Inês ChavesMargaret Ahmad
Mar 17, 2012·Proceedings of the National Academy of Sciences of the United States of America·Kiminori MaedaP J Hore
Sep 27, 2012·Journal of the American Chemical Society·Ilia A Solov'yovKlaus Schulten
Sep 18, 2013·Antioxidants & Redox Signaling·Nirakar SahooStefan H Heinemann
Jul 24, 2014·Scientific Reports·Richard MarleyAlex R Jones
Dec 31, 2014·Nature Methods·Jianyi YangYang Zhang
Apr 30, 2015·Chemical Reviews·Tomasz A WesolowskiXiuwen Zhou
Jul 22, 2015·Proceedings of the National Academy of Sciences of the United States of America·Harry B Gray, Jay R Winkler
Nov 18, 2015·Journal of Chemical Theory and Computation·James A MaierCarlos Simmerling
Mar 1, 2008·Journal of Chemical Theory and Computation·Berk HessErik Lindahl

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Citations

Oct 11, 2019·Journal of Biological Rhythms·Lauren E Foley, Patrick Emery
Mar 21, 2020·Frontiers in Physiology·Milena Damulewicz, Gabriella M Mazzotta

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