Coupling ion mobility separations, collisional activation techniques, and multiple stages of MS for analysis of complex peptide mixtures

Analytical Chemistry
C S Hoaglund-HyzerD E Clemmer

Abstract

An ion trap/ion mobility/quadrupole/collision cell/time-of-flight mass spectrometer that incorporates a differentially pumped orifice-skimmer cone region at the back of the drift tube has been developed for the analysis of peptide mixtures. The combined approach allows a variety of strategies to be employed for collisionally activating ions, and fragments can be monitored by subsequent stages of mass spectrometry in a parallel fashion, as described previously (Anal. Chem. 2000, 72, 2737). Here, we describe the overall experimental approach in detail. Applications involving different aspects of the initial mobility separation and various collisional activation and parallel sequencing strategies are illustrated by examining several simple peptide mixtures and a mixture of tryptic peptides from beta-casein. Detection limits associated with various experimental configurations and the utility for analysis of complex systems are discussed.

References

Oct 1, 1988·Biomedical & Environmental Mass Spectrometry·K Biemann
Jan 1, 1996·Analytical Chemistry·M Wilm, M Mann
Jun 13, 1998·Analytical Chemistry·C S HoaglundD E Clemmer
Aug 6, 1998·Journal of the American Society for Mass Spectrometry·A E CountermanD E Clemmer
Nov 18, 1991·Physical Review Letters·M F Jarrold, V A Constant
Sep 29, 1999·Analytical Chemistry·C A SrebalusD E Clemmer
Apr 11, 2000·Journal of the American Society for Mass Spectrometry·C A SrebalusD E Clemmer
Jul 25, 2000·Analytical Chemistry·C S Hoaglund-HyzerD E Clemmer
Feb 17, 2001·Journal of Mass Spectrometry : JMS·V H WysockiL A Breci
Feb 24, 2001·Analytical Chemistry·C S Hoaglund-Hyzer, D E Clemmer
Dec 26, 2001·Chemical Reviews·C S Hoaglund-HyzerD E Clemmer
Jul 1, 1997·Analytical Chemistry·Y Liu, D E Clemmer
Apr 1, 1986·Journal of Computational Chemistry·Scott J WeinerDavid A Case

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Citations

Jul 19, 2002·Journal of Mass Spectrometry : JMS·Gavin E Reid, Scott A McLuckey
Apr 8, 2011·Journal of the American Society for Mass Spectrometry·Zhiyu LiDavid E Clemmer
Sep 1, 2004·Journal of the American Society for Mass Spectrometry·Renã A SowellDavid E Clemmer
Oct 7, 2004·Journal of the American Society for Mass Spectrometry·Alexandre A ShvartsburgRichard D Smith
Mar 15, 2006·Journal of the American Society for Mass Spectrometry·Alexander Loboda
Nov 7, 2006·Journal of the American Society for Mass Spectrometry·Stormy L Koeniger, David E Clemmer
May 22, 2007·Journal of the American Society for Mass Spectrometry·Lei TaoDavid H Russell
Feb 7, 2009·Journal of the American Society for Mass Spectrometry·Ruwan T KurulugamaDavid E Clemmer
Nov 22, 2002·Journal of the American Society for Mass Spectrometry·David A BarnettRandy W Purves
Nov 5, 2002·Current Opinion in Chemical Biology·Wenjun Mo, Barry L Karger
Nov 30, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Young Jin LeeDavid E Clemmer
Jun 2, 2006·Analytical Chemistry·Alexandre A ShvartsburgRichard D Smith
Jan 16, 2007·Analytical Chemistry·Samuel I MerenbloomDavid E Clemmer
Jan 22, 2013·Analytical Chemistry·Hongli LiHerbert H Hill
Aug 8, 2008·Analytical Chemistry·Abu B KanuHerbert H Hill
Apr 1, 2011·The Journal of Physical Chemistry. B·Brian C BohrerDavid E Clemmer
Mar 23, 2011·Journal of Proteome Research·Stephen J ValentineDavid E Clemmer
Sep 1, 2011·International Journal for Ion Mobility Spectrometry : Official Publication of the International Society for Ion Mobility Spectrometry·Francisco Fernandez-LimaMelvin A Park
Nov 26, 2015·Journal of Chromatography. a·Michael A EwingDavid E Clemmer
Jul 3, 2010·International Journal of Mass Spectrometry·Yehia M IbrahimMikhail E Belov
Jul 2, 2008·Journal of Proteomics·Ruwan T KurulugamaDavid E Clemmer
Jan 18, 2008·Journal of Mass Spectrometry : JMS·Abu B KanuHerbert H Hill
Feb 24, 2007·Rapid Communications in Mass Spectrometry : RCM·Xiaoting TangHerbert H Hill
Sep 4, 2007·Rapid Communications in Mass Spectrometry : RCM·Houle WangDavid R Goodlett
Feb 27, 2016·Analytical Chemistry·A Bakarr Kanu, Anne Leal
Jun 15, 2011·Methods : a Companion to Methods in Enzymology·Sophie R HarveyPerdita E Barran
Jul 19, 2002·Journal of Mass Spectrometry : JMS
Dec 14, 2018·Chemical Science·Eva-Maria Schneeberger, Kathrin Breuker
Feb 3, 2006·The Journal of Physical Chemistry. a·Wes E SteinerHerbert H Hill
Dec 17, 2008·Analytical Chemistry·Francisco A Fernandez-LimaDavid H Russell
Dec 15, 2004·Analytical Chemistry·Alexandre A ShvartsburgRichard D Smith

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