Coupling Ion Specificity of the Flagellar Stator Proteins MotA1/MotB1 of Paenibacillus sp. TCA20.

Biomolecules
Sakura OnoeYoshiyuki Sowa

Abstract

The bacterial flagellar motor is a reversible rotary molecular nanomachine, which couples ion flux across the cytoplasmic membrane to torque generation. It comprises a rotor and multiple stator complexes, and each stator complex functions as an ion channel and determines the ion specificity of the motor. Although coupling ions for the motor rotation were presumed to be only monovalent cations, such as H+ and Na+, the stator complex MotA1/MotB1 of Paenibacillus sp. TCA20 (MotA1TCA/MotB1TCA) was reported to use divalent cations as coupling ions, such as Ca2+ and Mg2+. In this study, we initially aimed to measure the motor torque generated by MotA1TCA/MotB1TCA under the control of divalent cation motive force; however, we identified that the coupling ion of MotA1TCAMotB1TCA is very likely to be a monovalent ion. We engineered a series of functional chimeric stator proteins between MotB1TCA and Escherichia coli MotB. E. coli ΔmotAB cells expressing MotA1TCA and the chimeric MotB presented significant motility in the absence of divalent cations. Moreover, we confirmed that MotA1TCA/MotB1TCA in Bacillus subtilis ΔmotABΔmotPS cells generates torque without divalent cations. Based on two independent experimental results, we conclude th...Continue Reading

References

Apr 25, 2000·Biophysical Journal·X Chen, H C Berg
Apr 27, 2000·Journal of Bacteriology·L TurnerH C Berg
Mar 15, 2001·Biochimica Et Biophysica Acta·T Yorimitsu, M Homma
Dec 26, 2002·Annual Review of Biochemistry·Howard C Berg
Mar 12, 2003·Journal of Molecular Biology·Yukako AsaiMichio Homma
Jul 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Christopher V Gabel, Howard C Berg
Dec 2, 2004·Nature Reviews. Molecular Cell Biology·George H Wadhams, Judith P Armitage
Oct 7, 2005·Nature·Yoshiyuki SowaRichard M Berry
Jan 22, 2008·Journal of Molecular Biology·Yuichi InoueAkihiko Ishijima
Sep 18, 2008·Proceedings of the National Academy of Sciences of the United States of America·Naoya TeraharaMasahiro Ito
Sep 25, 2008·Quarterly Reviews of Biophysics·Yoshiyuki Sowa, Richard M Berry
Mar 6, 2010·Microbiology·Kai M Thormann, Anja Paulick
Jun 22, 2013·Proceedings of the National Academy of Sciences of the United States of America·Chien-Jung LoRichard M Berry
Feb 20, 2014·Proceedings of the National Academy of Sciences of the United States of America·Yoshiyuki SowaRichard M Berry
Jan 23, 2016·Scientific Reports·Riku ImazawaMasahiro Ito
Apr 9, 2017·Methods in Molecular Biology·Taishi Kasai, Yoshiyuki Sowa
Dec 12, 2018·Journal of Bacteriology·Shingo Nozaki, Hironori Niki
Jul 25, 2019·Biomolecules·Shuichi Nakamura, Tohru Minamino

❮ Previous
Next ❯

Citations

May 1, 2021·Frontiers in Microbiology·Anaïs Biquet-BisquertAshley L Nord
Jun 3, 2021·Biomolecules·Tohru Minamino, Keiichi Namba

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

TCA20
LabVIEW

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.