PMID: 698198Sep 5, 1978Paper

Coupling of a single adenylate cyclase to two receptors: adenosine and catecholamine

Biochemistry
A M Tolkovsky, A Levitzki

Abstract

A detailed kinetic analysis on the rate of activation of adenylate cyclase by 1-epinephrine and by adenosine, separately and combined, was performed. Both ligands were found to induce the activation of adenylate cyclase to its permanently active state in the presence of guanylyl imidodiphosphate (GppNHp). The activation followed strictly first-order kinetics. On the basis of these experiments, it was found that all of the enzyme pool can be activated by the beta-adrenergic receptor, but only 60 to 70% of the enzyme can also be activated by an adenosine receptor. The remaining 30 to 40% cannot be activated by adenosine. While previous experiments have led us to conclude that the epinephrine receptor is uncoupled from the adenylate cyclase, it seems that the adenosine receptor is either precoupled to the enzyme or forms a long-lived intermediate of adenosing-receptor-enzyme complex. From the pattern of enzyme activation by the two ligands and GppNHp, it may be concluded that the two ligands, adenosine and the beta-agonist, activate the adenylate cyclase through a common guanyl nucleotide regulatory site. This assertion is supported by the finding that both adenosine and 1-epinephrine, in the presence of GTP, induce the reversal o...Continue Reading

References

Dec 8, 1976·Biochimica Et Biophysica Acta·D Cassel, Z Selinger
Feb 7, 1977·Biochemical and Biophysical Research Communications·A Levitzki
Jan 1, 1976·Journal of Supramolecular Structure·A LevitzkiM L Steer
Apr 1, 1974·Analytical Biochemistry·Y SalomonM Rodbell

❮ Previous
Next ❯

Citations

Dec 3, 2008·Naunyn-Schmiedeberg's Archives of Pharmacology·Peter Hein, Moritz Bünemann
Jan 1, 1993·Life Sciences·A LevitzkiA Bar-Sinai
Jan 1, 1991·Pharmacology & Therapeutics·A Levitzki, A Bar-Sinai
Jun 1, 1988·Molecular and Cellular Endocrinology·M B Anand-Srivastava
Oct 1, 1995·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·C I TascaD O Souza
Sep 15, 1994·European Journal of Pharmacology·J C MakP J Barnes
Jan 22, 2009·IET Systems Biology·M R MauryaS Subramaniam
Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·T B NielsenM Rodbell
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·Y I HenisE J Helmreich
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A KirkwoodJ E Lisman
Jan 1, 1997·British Journal of Clinical Pharmacology·D C BussP A Routledge
Jan 1, 1988·Cell Biophysics·A Levitzki
Nov 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Scott J BornheimerShankar Subramaniam
Jun 12, 1985·Biochimica Et Biophysica Acta·A Levitzki
Feb 12, 2008·Trends in Pharmacological Sciences·Martin J LohseMoritz Bünemann
Feb 5, 2008·British Journal of Pharmacology·J Zezula, M Freissmuth
Jun 1, 1983·Archives of Biochemistry and Biophysics·M B Anand-Srivastava, M Cantin
Dec 1, 1985·Archives of Biochemistry and Biophysics·M B Anand-Srivastava
Jul 1, 1989·British Journal of Clinical Pharmacology·D C BussJ H Lazarus
Jan 1, 1980·Annals of the New York Academy of Sciences·J A ZadunaiskyB Cherksey
Oct 1, 1980·Journal of Neurochemistry·M B Anand-Srivastava, R A Johnson
Jul 1, 1985·Archives of Biochemistry and Biophysics·B BaronM Sokolovsky
Mar 23, 1978·Nature·D Atlas, A Levitzki
May 12, 1988·Nature·E J Neer, D E Clapham
Jan 26, 2008·The Journal of Biological Chemistry·Christoforos CharalambousJürgen Zezula
Jul 26, 1988·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·C P DownesT K Harden

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adrenergic Receptors: Trafficking

Adrenergic receptor trafficking is an active physiological process where adrenergic receptors are relocated from one region of the cell to another or from one type of cell to another. Discover the latest research on adrenergic receptor trafficking here.