Coupling of aerobic metabolism to active ion transport in the kidney

The Journal of Physiology
R S Balaban, L J Mandel

Abstract

1. The redox state of mitochondrial NAD was monitored fluorometrically as a function of active ion transport work in the isolated doubly perfused bullfrog kidney. 2. Initial experiments to measure the O2 consumption (QO2) of small pieces from the bullfrog kidney gave a basal QO2 - 3.0 (+/- 0.43) nmoles O2/mg dry wt. min. Addition of 50 microM-ouabain inhibited QO2 by 72.7%. Subsequent addition of the mitochondrial uncoupler 1799 stimulated QO2 by 226%, while cyanide totally inhibited respiration. 3. Ion transport functional parameters and NADH fluorescence were simultaneously monitored during systematic reductions in perfusate PO2 to test the sufficiency of O2 delivery to the isolated perfused frog kidney. No significant changes in transport functions or fluorescence were observed until the PO2 dropped to 184 mm Hg or below. O2 tensions of 184 mm Hg or below caused decreases in G.F.R. and transport functions which were accompanied by an increase in NADH fluorescence. The lack of changes in kidney function in the PO2 range 550-340 mmHg suggested that the tissue is adequately oxygenated at the normal perfusate PO2 of 550 mmHg. 4. The relationship between active transport rate and NAD redox levels was studied by increasing transpo...Continue Reading

Citations

Dec 15, 2012·Annals of Biomedical Engineering·Kevin StaniszewskiMahsa Ranji
Jan 1, 1983·Critical Reviews in Clinical Laboratory Sciences·A D Macknight, J P Leader
Feb 5, 2015·The New England Journal of Medicine·Kamel S Kamel, Mitchell L Halperin
Sep 8, 2016·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Kamel S KamelMitchell L Halperin
Sep 28, 2004·Journal of Biomedical Optics·Avraham MayevskyAlex Jaronkin
Mar 17, 2005·American Journal of Physiology. Renal Physiology·Xiaoming ZhouMaurice B Burg
Sep 1, 2006·American Journal of Physiology. Cell Physiology·Avraham Mayevsky, Gennady G Rogatsky
Nov 2, 2019·Nature Reviews. Nephrology·Kenneth M RaltoSamir M Parikh
Oct 6, 2020·Frontiers in Physiology·Koji TakemuraReiko Inagi
Mar 1, 1990·The American Journal of Physiology·R S Balaban

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