Coupling of calcium receptors to inositol phosphate and cyclic AMP generation in mammalian cells and Xenopus laevis oocytes and immunodetection of receptor protein by region-specific antipeptide antisera

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
W ChangDolores M Shoback

Abstract

Ca2+ and other divalent cations modulate parathyroid hormone secretion by interacting with cell-surface Ca2+-sensing receptors (CaRs). We assessed the ability of these receptors to couple to Ca2+ mobilization, inositol phosphate (InsP) accumulation, and cyclic AMP production in different expression systems. In Xenopus laevis oocytes injected with bovine parathyroid CaR cRNA, the addition of extracellular cations to 1.5 mM Ca2+, 5.5 mM Mg2+, or 10 microM Gd3+ significantly increased 45Ca efflux (p < 0.01). InsP accumulation also increased dramatically when adding these cations to human embryonic kidney (HEK) 293 cells stably transfected with wild-type bovine parathyroid CaR cDNA. Raising the extracellular [Ca2+] ([Ca2+]o) from 0.1 to > 1.4 mM in oocytes and to > 1.0 mM in HEK 293 cells stimulated significant increments in 45Ca efflux and InsP accumulation, respectively (p < 0.05). In contrast, Ca2+ and Mg2+ increased InsPs to a lesser extent in COS 7 cells transiently transfected with CaR cDNA. In HEK 293 cells stably expressing CaR cDNA, there were significant reductions in cAMP content when adding high Ca2+, Mg2+, Gd3+, or the CaR modulator NPS R-467. Three region-specific anti-CaR peptide antisera immunoblotted bands of appro...Continue Reading

References

Jan 1, 1992·Methods in Enzymology·Y Oron, N Dascal
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M M White, T Reisine
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R E StraubM C Gershengorn
Jan 1, 1987·Methods in Enzymology·D A Melton
Feb 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T H ChenD M Shoback
Apr 11, 1995·Proceedings of the National Academy of Sciences of the United States of America·M RuatS H Snyder
May 26, 1995·The Journal of Biological Chemistry·J E GarrettF Fuller
Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·D RiccardiS C Hebert
Jul 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·D M ShobackR M Johnson
Apr 15, 1993·Nature
Jan 1, 1996·Annual Review of Pharmacology and Toxicology·T GudermannG Schultz
May 1, 1997·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·O KiforE M Brown
Jun 13, 1997·The Journal of Biological Chemistry·S G Rhee, Y S Bae

❮ Previous
Next ❯

Citations

Jan 29, 2008·Nature Neuroscience·Thomas N VizardAlun M Davies
Aug 24, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M PiL d Quarles
Jun 18, 2004·Cell Calcium·Wenhan Chang, Dolores Shoback
Jun 18, 2004·Cell Calcium·Jianming Ba, Peter A Friedman
Jan 12, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Giovanna ValentiMaria Svelto
May 25, 2012·Kidney International·Silvia FerrèRené J M Bindels
May 25, 2012·The Journal of Investigative Dermatology·Chia-Ling TuDaniel D Bikle
Dec 22, 2015·Seminars in Cell & Developmental Biology·Christian Santa MariaWenhan Chang
Nov 9, 2004·Experimental Cell Research·Brian JensenKamil A Akanbi
Jun 16, 2009·General and Comparative Endocrinology·Christopher A LoretzYoshio Takei
Oct 1, 2011·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Melita M Dvorak-EwellDolores M Shoback
Sep 9, 2005·The Journal of Biological Chemistry·Caroline SilveMartial Ruat
Mar 31, 1999·Endocrinology·W ChangD Shoback
Feb 23, 2008·Annals of Medicine·Kirsten Y RenkemaJoost G Hoenderop
Sep 10, 2019·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Zhiqiang ChengWenhan Chang
Jul 10, 2002·Current Opinion in Nephrology and Hypertension·Thorsten Vetter, Martin J Lohse
Aug 24, 2010·Endocrine Reviews·Aaron L MagnoThomas Ratajczak
Aug 29, 1998·The Journal of Biological Chemistry·Y OdaD D Bikle
Feb 24, 2001·American Journal of Physiology. Renal Physiology·O KiforE M Brown
Feb 1, 2012·Clinical Kidney Journal·John CunninghamPiergiorgio Messa
Sep 4, 2003·American Journal of Physiology. Renal Physiology·Jianming BaPeter A Friedman
Jun 19, 2001·American Journal of Physiology. Endocrinology and Metabolism·W ChangD Shoback
Jun 14, 2019·Frontiers in Pharmacology·Emiel P C van der VorstYvonne Döring
Jan 12, 2001·Physiological Reviews·E M Brown, R J MacLeod
Sep 20, 2006·Cancer Research·Jinhui LiaoLaurie K McCauley
Apr 17, 2021·ACS Pharmacology & Translational Science·Jiayin DiaoKatie Leach
Apr 27, 2002·Current Problems in Surgery·Barbara K Kinder, Andrew F Stewart
Jan 12, 2000·Molecular and Cellular Endocrinology·W ChangD Shoback

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.