Coupling of cAMP/PKA and MAPK signaling in neuronal cells is dependent on developmental stage

Experimental Neurology
D M Vogt WeisenhornB H Wainer

Abstract

Neurite formation, an essential feature of neuronal development, is believed to involve participation of the ras-mitogen-activated protein kinase (MAPK) and cAMP-dependent protein kinase A (cAMP/PKA)-mediated signaling pathways. These pathways have been studied extensively in the rat pheochromocytoma cell line PC12, and current hypotheses suggest a single effector mechanism resulting from the convergence of cAMP/PKA and MAPK signaling. However, based on observations using a central neuronal progenitor cell line, AS583-8, there also exists evidence that the two signaling pathways may act independently resulting in neurites with differing dynamic features. In the present study, the upstream components of cAMP/PKA signaling were examined in AS583-8 cells as well as possible interactions with the MAPK pathway. We found that activation of PKA is both necessary and sufficient for the elaboration of rapidly forming processes, typical of the cAMP response. In addition, blockade of the MAPK pathway has no effect on the cAMP response, suggesting that activation of the cAMP/PKA pathway can stimulate neurite formation independent of the MAPK pathway. In order to evaluate which cell line model, PC12 vs AS583-8, best reflects the signaling f...Continue Reading

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Citations

Dec 21, 2006·Journal of Cellular Biochemistry·Chien-Hsing ChenSeu-Mei Wang
Jun 3, 2010·Journal of Cellular Biochemistry·Kohtaro MiyazawaLaura Manuelidis
Aug 2, 2002·Biochemical and Biophysical Research Communications·María Leonor Quintero-MoraBulmaro Cisneros
Oct 13, 2005·Chemico-biological Interactions·Ze-Jian WangYang Lu
Aug 16, 2005·Cellular Signalling·Mien-Sheng ChuShih-Chieh Hung
Oct 30, 2019·Cellular and Molecular Life Sciences : CMLS·Sarah A BennisonKazuhito Toyo-Oka
Jun 21, 2002·Trends in Cell Biology·Philip J S Stork, John M Schmitt
May 20, 2004·Experimental Cell Research·Rosalinda AcostaBulmaro Cisneros

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