Coupling of DNA binding and helicase activity is mediated by a conserved loop in the MCM protein

Nucleic Acids Research
Nozomi SakakibaraZvi Kelman

Abstract

Minichromosome maintenance (MCM) helicases are the presumptive replicative helicases, thought to separate the two strands of chromosomal DNA during replication. In archaea, the catalytic activity resides within the C-terminal region of the MCM protein. In Methanothermobacter thermautotrophicus the N-terminal portion of the protein was shown to be involved in protein multimerization and binding to single and double stranded DNA. MCM homologues from many archaeal species have highly conserved predicted amino acid similarity in a loop located between beta7 and beta8 in the N-terminal part of the molecule. This high degree of conservation suggests a functional role for the loop. Mutational analysis and biochemical characterization of the conserved residues suggest that the loop participates in communication between the N-terminal portion of the helicase and the C-terminal catalytic domain. Since similar residues are also conserved in the eukaryotic MCM proteins, the data presented here suggest a similar coupling between the N-terminal and catalytic domain of the eukaryotic enzyme.

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Citations

Dec 17, 2008·Proceedings of the National Academy of Sciences of the United States of America·Aaron S BrewsterXiaojiang S Chen
Jan 24, 2009·Proceedings of the National Academy of Sciences of the United States of America·Elizabeth R BarryStephen D Bell
Aug 9, 2011·Nucleic Acids Research·Miao PanZvi Kelman
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Jan 29, 2011·Extremophiles : Life Under Extreme Conditions·Nozomi SakakibaraZvi Kelman
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Jan 16, 2009·Biochemical Society Transactions·Nozomi SakakibaraZvi Kelman
Dec 2, 2020·Open Biology·Patricia Pérez-ArnaizThorsten Allers
Feb 27, 2009·Biochemistry·Nozomi SakakibaraZvi Kelman

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Datasets Mentioned

BETA
CAI50644

Methods Mentioned

BETA
Fluorescence
differential
circular dichroism
Gel-filtration
gel filtration
filter binding

Software Mentioned

BLAST
PyMol
EXAM
MUSCLE
PHYLIP promlk
Erithacus
Jalview
GraFit

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