Coupling of fully automated chip-based electrospray ionization to high-capacity ion trap mass spectrometer for ganglioside analysis

Analytical Biochemistry
Reinaldo AlmeidaAlina D Zamfir

Abstract

NanoMate robot was coupled to a high-capacity ion trap (HCT) mass spectrometer to create a system merging automatic chip-based electrospray ionization (ESI) infusion, ultrafast ion detection, and multistage sequencing at superior sensitivity. The interface between the NanoMate and HCT mass spectrometer consists of an in-laboratory constructed mounting device that allows adjustment of the robot position with respect to the mass spectrometer inlet. The coupling was optimized for ganglioside (GG) high-throughput analysis in the negative ion mode and was implemented in clinical glycolipidomics for identification and structural characterization of anencephaly-associated species. By NanoMate HCT mass spectrometry (MS), data corroborating significant differences in GG expression in anencephalic versus age-matched normal brain tissue were collected. The feasibility of chip-based nanoESI HCT multistage collision-induced dissociation (CID MS(n)) for polysialylated GG fragmentation and isomer discrimination was tested on a GT1 (d18:1/18:0) anencephaly-associated structure. MS(2)-MS(4) obtained by accumulating scans at variable fragmentation amplitudes gave rise to the first fragmentation patterns from which the presence of GT1b structural...Continue Reading

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Citations

Jul 24, 2013·Analytical and Bioanalytical Chemistry·Alina D ZamfirCorina Flangea
Jan 21, 2012·Journal of Lipid Research·Alicia L RichardsSarah Trimpin
Oct 1, 2011·Biochimica Et Biophysica Acta·Corina FlangeaAlina D Zamfir
Jun 28, 2011·Biochimica Et Biophysica Acta·Corina FlangeaAlina D Zamfir
Jul 6, 2010·Clinical Biochemistry·Michael Vogeser, Fabian Kirchhoff
Jan 3, 2013·Journal of Mass Spectrometry : JMS·Alina F SerbAlina D Zamfir
Jan 9, 2014·Mass Spectrometry Reviews·Xiaojun FengXin Liu
Apr 19, 2016·Analytical Chemistry·Mirela SarbuAlina D Zamfir
Jun 18, 2016·Analytical Biochemistry·Adrian C RobuAlina D Zamfir
Jan 22, 2020·Journal of Mass Spectrometry : JMS·Raluca IcaAlina D Zamfir
Feb 4, 2011·Bioanalytical Reviews·Tobias Kind, Oliver Fiehn
Apr 20, 2020·Clinica Chimica Acta; International Journal of Clinical Chemistry·Michal AlexovičJán Sabo
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Apr 27, 2021·Journal of the American Society for Mass Spectrometry·Mirela SarbuAlina D Zamfir
Aug 14, 2013·Analytical Chemistry·Tadahiro KumagaiKazuhiro Aoki

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