Coupling of Integrin α5 to Annexin A2 by Flow Drives Endothelial Activation.

Circulation Research
Chenghu ZhangDing Ai

Abstract

Atherosclerosis preferentially occurs at specific sites of the vasculature where endothelial cells (ECs) are exposed to disturbed blood flow. Translocation of integrin α5 to lipid rafts promotes integrin activation and ligation, which is critical for oscillatory shear stress (OSS)-induced EC activation. However, the underlying mechanism of OSS promoted integrin α5 lipid raft translocation has remained largely unknown. The objective of this study was to specify the mechanotransduction mechanism of OSS-induced integrin α5 translocation and subsequent EC activation. Mass spectrometry studies identified endothelial ANXA2 (annexin A2) as a potential carrier allowing integrin α5β1 to traffic in response to OSS. Interference by siRNA of AnxA2 in ECs greatly decreased OSS-induced integrin α5β1 translocation to lipid rafts, EC activation, and monocyte adhesion. Pharmacological and genetic inhibition of PTP1B (protein tyrosine phosphatase 1B) blunted OSS-induced integrin α5β1 activation, which is dependent on Piezo1-mediated calcium influx in ECs. Furthermore, ANXA2 was identified as a direct substrate of activated PTP1B by mass spectrometry. Using bioluminescence resonance energy transfer assay, PTP1B-dephosphorylated ANXA2 at Y24 was f...Continue Reading

References

Feb 1, 1995·Molecular Biology of the Cell·J M Lewis, M A Schwartz
Nov 7, 1998·The Journal of Biological Chemistry·I S BhullarS Chien
Mar 28, 2002·Physiological Reviews·Volker Gerke, Stephen E Moss
Oct 18, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Yong Chool BooHanjoong Jo
Apr 29, 2004·American Journal of Physiology. Renal Physiology·David B N LeeIrene L Hale
May 12, 2004·The Journal of Experimental Medicine·Sucharita SenBanerjeeMukesh K Jain
Jun 1, 2004·Journal of Cell Science·Ursula Rescher, Volker Gerke
Apr 6, 2005·The Journal of Cell Biology·A Wayne OrrMartin Alexander Schwartz
Jun 2, 2005·Nature Reviews. Molecular Cell Biology·Volker GerkeStephen E Moss
Feb 4, 2006·Molecular Cell·Tiila KiemaDavid A Calderwood
Aug 25, 2006·Molecular Biology of the Cell·A Wayne OrrMartin A Schwartz
Feb 19, 2008·The Journal of Biological Chemistry·Janice M ZabolotnyBarbara B Kahn
Feb 7, 2009·Nature Reviews. Molecular Cell Biology·Cornelia Hahn, Martin A Schwartz
Oct 22, 2009·The Journal of Biological Chemistry·Nicholas J AnthisIain D Campbell
Sep 13, 2011·Methods in Molecular Biology·Guanyuan FuBing-Hao Luo
Sep 29, 2011·Nature Cell Biology·Juha K RantalaJohanna Ivaska
Aug 22, 2012·The Journal of Cell Biology·Stephanie L GuptonFrank B Gertler
Mar 23, 2013·International Journal of Molecular Sciences·Alamelu BharadwajDavid Waisman
Aug 2, 2014·The Journal of Clinical Investigation·Petrus R de JongEyal Raz
Dec 24, 2014·Proceedings of the National Academy of Sciences of the United States of America·Juanfei WangWei Guo
Jan 7, 2016·Proceedings of the National Academy of Sciences of the United States of America·Xiaoli SunYi Zhu
Sep 7, 2016·Nature Cell Biology·Sanguk YunMartin A Schwartz
Sep 28, 2016·Proceedings of the National Academy of Sciences of the United States of America·Kuei-Chun WangShu Chien
Nov 1, 2016·The Journal of Clinical Investigation·ShengPeng WangStefan Offermanns
Mar 24, 2018·Biochimica Et Biophysica Acta. Molecular Cell Research·Juan C López-RodríguezJavier Turnay
Sep 9, 2018·The Journal of Experimental Medicine·Julián Albarrán-JuárezStefan Offermanns
Jan 6, 2019·Biochimica Et Biophysica Acta. Molecular Cell Research·Marion GabelSylvette Chasserot-Golaz
Jan 11, 2019·The Journal of Clinical Investigation·Bochuan LiDing Ai

❮ Previous
Next ❯

Citations

Sep 25, 2020·Circulation Research·Catherine DemosHanjoong Jo
Aug 6, 2020·International Journal of Molecular Sciences·Daniel Lietha, Tina Izard
Nov 29, 2020·Free Radical Biology & Medicine·Jinna ChenDangheng Wei
Feb 26, 2021·Arteriosclerosis, Thrombosis, and Vascular Biology·Zhan HuJiangping Song
Feb 25, 2021·Clínica e investigación en arteriosclerosis : publicación oficial de la Sociedad Española de Arteriosclerosis·Nerea Méndez-BarberoLuis Miguel Blanco-Colio
Mar 20, 2021·Frontiers in Cell and Developmental Biology·Han BaoYing-Xin Qi
Apr 4, 2021·Biomedicines·Thijs J SluiterMargreet R de Vries
Apr 4, 2021·International Journal of Molecular Sciences·Thomas GrewalUrsula Rescher
Jul 3, 2021·International Journal of Molecular Sciences·Hana I Lim, Katherine A Hajjar
Jul 19, 2021·Clínica e investigación en arteriosclerosis : publicación oficial de la Sociedad Española de Arteriosclerosis·José Martínez-González, Irene Corrales
Nov 9, 2021·Circulation Research·Shu-Yi WeiJeng-Jiann Chiu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

ApoE Phenotypes

Apolipoprotein E (APOE) is a protein involved in fat metabolism and associated with the pathogenesis of Alzheimer's disease and cardiovascular disease. Here is the latest research on APOE phenotypes.

Atherosclerosis Disease Progression

Atherosclerosis is the buildup of plaque on artery walls, causing stenosis which can eventually lead to clinically apparent cardiovascular disease. Find the latest research on atherosclerosis disease progression here.