PMID: 11316267Apr 24, 2001Paper

Coupling of L-type calcium channels to neurotransmitter release at mouse motor nerve terminals

Pflügers Archiv : European journal of physiology
Francisco J UrbanoO D Uchitel

Abstract

Previously, we have presented evidence for the presence of L-type voltage-dependent Ca2+ channels (VDCC) in 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid, (acetoxymethyl)ester (BAPTA-AM)-incubated motor nerve terminals (MNTs) of the levator auris muscle of mature mice. The aim of the present work was to study the coupling of these L-type VDCC to neurotransmitter release by inhibiting protein phosphatases. We thus studied the effects of the protein phosphatase inhibitors okadaic acid (OA) and pervanadate on quantal content (QC) of transmitter release with the P/Q-type channels fully blocked. The QC was not significantly different under the three experimental conditions tested: incubation with dimethylsulphoxide (DMSO), ethylene-glycol-bis(beta-aminoethylether)-N,N,N',N'-tetraacetic acid, (acetoxymethyl)ester (EGTA-AM) and BAPTA-AM. After preincubation with OA (1 microM), but not with pervanadate, QC increased substantially in the BAPTA-AM-incubated (up to 400%) MNT, but not in those incubated with DMSO or EGTA-AM. The OA-induced increment of QC was attenuated greatly (approximately 95% reduction) by preincubation with either nitrendipine (10 microM) or calciseptine (300 nM). The effect of OA (1 microM) and pervanadate...Continue Reading

Citations

Jun 3, 2006·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Ehud OhanaMichal Hershfinkel
Sep 15, 2012·Bulletin of Experimental Biology and Medicine·A E GaydukovO P Balezina
Sep 10, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Javier Zorrilla de San MartínEleonora Katz
Jul 19, 2011·Journal of Physiology, Paris·Ana Belén Elgoyhen, Eleonora Katz
Aug 6, 2008·Journal of Neuroimmunology·Francisco J UrbanoOsvaldo D Uchitel
Jul 29, 2008·Peptides·Richard C Rogers, Gerlinda E Hermann
Nov 1, 2003·Annals of the New York Academy of Sciences·Silvana NudlerOsvaldo D Uchitel
Mar 14, 2008·The European Journal of Neuroscience·Paula P PerissinottiOsvaldo D Uchitel
Aug 24, 2010·The European Journal of Neuroscience·Paula P Perissinotti, Osvaldo D Uchitel
Feb 7, 2012·Experimental Neurology·Pablo BaliñoCarlos M G Aragon
Dec 19, 2014·Frontiers in Systems Neuroscience·Eleonora Katz, Ana Belén Elgoyhen
Dec 21, 2002·Synapse·Jean Vautrin, Jeffery L Barker
Feb 28, 2003·The Journal of Pharmacology and Experimental Therapeutics·Michael T Flink, William D Atchison
Oct 29, 2015·The Journal of Biological Chemistry·Meng-Hua ZhouHui-Lin Pan
Sep 10, 2018·Biochemistry. Biokhimii︠a︡·E O TarasovaO P Balezina
Nov 9, 2016·Doklady Biological Sciences : Proceedings of the Academy of Sciences of the USSR, Biological Sciences Sections·V F KhuzakhmetovaE E Nikolsky
Dec 5, 2014·The Journal of Pharmacology and Experimental Therapeutics·Elizabeth Molina-CamposWilliam D Atchison

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