Coupling of oxytocin receptor to G proteins in rat myometrium during labor: Gi receptor interaction

The American Journal of Physiology
Z Strakova, M S Soloff

Abstract

Occupancy of oxytocin receptor (OTR) binding sites in pregnant rat myometrial membranes with iodinated oxytocin antagonist (OTA), followed by detergent solubilization and size selection, showed that radioactivity eluted in two distinct peaks: one corresponding in size to the isolated receptor (approximately 60 kDa) and the other ranging from 240 to 320 kDa. The unliganded 240- to 320-kDa fraction contained OTRs coupled to G proteins, as the addition of oxytocin (OT) increased guanosine 35S-labeled 5'-O-(3-thiotriphosphate) binding up to twofold in a dose-dependent manner. The effects of OT were blocked by coincubation with OTA. G protein alpha-subunits associated with OTRs in the 240- to 320-kDa peak were identified by immunoadsorption. Significant amounts of both G alpha q/11 and G alpha i3 were associated with the OTR; a lesser amount of G alpha s was complexed. Using the same approach but with antibodies to effector enzymes, we observed that phospholipase C beta 1 (PLC beta 1) and PLA2 were also associated with the OTR. The results corroborate the well-established interaction of OTR with Gq and further show that Gi coupling might be an important component of OTR signal transduction. To further investigate the interaction of ...Continue Reading

References

Jun 22, 1979·Science·M S SoloffM J Fernstrom
Apr 9, 1992·Nature·T KimuraH Okayama
Oct 1, 1988·American Journal of Obstetrics and Gynecology·M P SchreyP J Steer
Jan 5, 1996·The Journal of Biological Chemistry·J R HeplerA G Gilman

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Citations

Mar 29, 2001·Physiological Reviews·G Gimpl, F Fahrenholz
Dec 7, 2006·American Journal of Physiology. Endocrinology and Metabolism·Masaki KawamataKatsuhiko Nishimori
Sep 7, 2006·American Journal of Physiology. Endocrinology and Metabolism·Dominic Devost, Hans H Zingg
Jun 14, 2018·Physiological Reviews·Benjamin Jurek, Inga D Neumann
Nov 17, 2020·Frontiers in Endocrinology·Beatrix StadlerRalf Middendorff
Aug 4, 2021·ACS Chemical Neuroscience·Antonio Espinosa de Los Monteros-ZúñigaAbimael González-Hernández

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