Coupling of proton source and sink via H+-migration along the membrane surface as revealed by double patch-clamp experiments

FEBS Letters
Y N Antonenko, P Pohl

Abstract

Long-range proton transfer along the surface of black lipid bilayers was observed between two integral membrane channels (gramicidins), one operating as a proton source, the other as a sink, by patch-clamp technique. In contrast, potassium ions were shown to equilibrate with the aqueous bulk phase before being consumed. Both channels opened and closed simultaneously only if the charge between them was carried by protons. In this case an anomalous high conductance between two patched membrane fragments was measured, each of them containing one single gramicidin channel. The coupled state disappeared when the distance between these two channels was increased above the critical value. The latter was shown to increase with the channel lifetime. Our results support the idea of the 'localized' proton coupling, in which protons that have been pumped across membranes migrate along the membrane surface to reach another membrane protein that utilizes the established pH gradient.

References

Apr 1, 1988·Biophysical Journal·A Ring, J Sandblom
Aug 1, 1966·Biological Reviews of the Cambridge Philosophical Society·P Mitchell
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·T H Haines
Dec 30, 1981·Biochimica Et Biophysica Acta·H Tedeschi
Aug 24, 1995·Nature·R J Williams
Jan 17, 1995·Proceedings of the National Academy of Sciences of the United States of America·U AlexievM P Heyn
Jul 29, 1994·Biochimica Et Biophysica Acta·B GabrielJ Teissié
Jul 1, 1993·Trends in Biochemical Sciences·J TeissiéM Prats

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Citations

Mar 23, 2012·Journal of Bioenergetics and Biomembranes·Toshikazu HirabayashiIsao Yumoto
Dec 26, 2001·Biochimica Et Biophysica Acta·Vitali BorisenkoG Andrew Woolley
May 16, 2000·Chemistry and Physics of Lipids·M Langner, K Kubica
Apr 21, 2006·Biochimica Et Biophysica Acta·Armen Y MulkidjanianDmitry A Cherepanov
Apr 12, 2016·Scientific Reports·Tobias NilssonPeter Brzezinski
Oct 29, 2000·Biophysical Journal·S M SaparovP Pohl
May 23, 2002·Biophysical Journal·Yuri GeorgievskiiAlexei A Stuchebrukhov
Jan 28, 2003·Biophysical Journal·Steffen SerowyPeter Pohl
Nov 29, 2005·Journal of Bioscience and Bioengineering·Toshitaka GotoIsao Yumoto
Jun 9, 2006·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Armen Y Mulkidjanian, Dmitry A Cherepanov
Jul 19, 2011·Journal of Bioenergetics and Biomembranes·Toshihede MatsunoIsao Yumoto
Feb 4, 2014·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Dong Hoon SongAnn Marie Sastry
May 27, 2011·Journal of Physics. Condensed Matter : an Institute of Physics Journal·E S Medvedev, A A Stuchebrukhov
Jun 9, 2017·Scientific Reports·Johannes SjöholmPeter Brzezinski
Jun 19, 2009·The Journal of Physical Chemistry. B·Markus S MiettinenMikko Karttunen
May 17, 2000·Biochimica Et Biophysica Acta·J Heberle

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