Coupling of receptor interference and a host-dependent post-binding entry deficiency in a gammaretroviral envelope protein.

Retrovirology
Shervin BahramiFinn Skou Pedersen

Abstract

SL3-2 is a unique polytropic murine gammaretroviral isolate that is only able to infect murine cells. We have previously shown that two mutations R212G and T213I located on the surface of the receptor binding domain in a region designated the VR3 loop can alter the species tropism of this envelope protein. This location suggests that the VR3 loop composition has an influence on receptor interaction and thereby affects binding as well as superinfection resistance. In order to investigate this further, we have studied the binding and interference patterns of the SL3-2 envelope and its mutants. We find unexpectedly that wild type SL3-2 envelope binds equally well to both permissive and non-permissive cells, indicating a post binding defect when interacting with the human Xpr1. Using replication competent viruses containing envelopes from SL3-2 or its mutants we find that the same amino acid mutations can dramatically alter the interference profile of this polytropic ENV, suggesting that the same amino acid changes that cause the post binding defect also influence interaction with the receptor. The envelope protein of SL3-2 MLV shows an entry defect on non-murine cells. This is coupled to a dramatically reduced ability to interfere...Continue Reading

References

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Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·M P KavanaughA D Miller
Feb 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·C S TailorD Kabat
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Jan 29, 2003·Journal of Virology·Anna L BarnettJames M Cunningham
Dec 13, 2003·Journal of Virology·Tatiana ZavorotinskayaLorraine M Albritton
Dec 13, 2003·Journal of Virology·Zhaohui Qian, Lorraine M Albritton
Aug 17, 2004·Journal of Virology·Shervin BahramiFinn Skou Pedersen
Aug 19, 2005·Retrovirology·Micha NetheAntoinette C van der Kuyl

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Methods Mentioned

BETA
FACS
PCR
flow cytometry

Software Mentioned

Rasmol

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