Coupling of Retrograde Flow to Force Production During Malaria Parasite Migration

ACS Nano
Katharina A QuadtFriedrich Frischknecht

Abstract

Migration of malaria parasites is powered by a myosin motor that moves actin filaments, which in turn link to adhesive proteins spanning the plasma membrane. The retrograde flow of these adhesins appears to be coupled to forward locomotion. However, the contact dynamics between the parasite and the substrate as well as the generation of forces are complex and their relation to retrograde flow is unclear. Using optical tweezers we found retrograde flow rates up to 15 μm/s contrasting with parasite average speeds of 1-2 μm/s. We found that a surface protein, TLP, functions in reducing retrograde flow for the buildup of adhesive force and that actin dynamics appear optimized for the generation of force but not for maximizing the speed of retrograde flow. These data uncover that TLP acts by modulating actin dynamics or actin filament organization and couples retrograde flow to force production in malaria parasites.

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Citations

May 10, 2016·Trends in Parasitology·Axel Hochstetter, Thomas Pfohl
Aug 31, 2016·The Journal of Cell Biology·Isabelle Tardieux, Jake Baum
Nov 29, 2016·Nature Reviews. Microbiology·Mariana De NizVolker T Heussler
Jul 15, 2016·PLoS Pathogens·Kartik S BaneFriedrich Frischknecht
Feb 18, 2017·Molecular Biology of the Cell·Rachel V StadlerWilliam H Guilford
Jan 25, 2017·ELife·Dennis Klug, Friedrich Frischknecht
Sep 5, 2017·Nature Reviews. Microbiology·Karine FrénalDominique Soldati-Favre
Jun 25, 2019·PLoS Biology·Simon GrasMarkus Meissner
Dec 24, 2019·Chembiochem : a European Journal of Chemical Biology·Tuntun WangDong Kee Yi
Feb 9, 2020·Journal of Cell Science·Catherine A MoreauFriedrich Frischknecht
May 26, 2018·Parasitology Research·Henriette PrinzFriedrich Frischknecht
May 30, 2017·PLoS Pathogens·Catherine A MoreauFriedrich Frischknecht
Jan 22, 2017·Cold Spring Harbor Perspectives in Medicine·Friedrich Frischknecht, Kai Matuschewski
Apr 23, 2021·Cellular Microbiology·Sujaan DasJavier Periz

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