Covalent and stable CuAAC modification of silicon surfaces for control of cell adhesion

Chembiochem : a European Journal of Chemical Biology
Surendra VuttiMorten Meldal

Abstract

Stable primary functionalization of metal surfaces plays a significant role in reliable secondary attachment of complex functional molecules used for the interfacing of metal objects and nanomaterials with biological systems. In principle, this can be achieved through chemical reactions either in the vapor or liquid phase. In this work, we compared these two methods for oxidized silicon surfaces and thoroughly characterized the functionalization steps by tagging and fluorescence imaging. We demonstrate that the vapor-phase functionalization only provided transient surface modification that was lost on extensive washing. For stable surface modification, a liquid-phase method was developed. In this method, silicon wafers were decorated with azides, either by silanization with (3-azidopropyl)triethoxysilane or by conversion of the amine groups of an aminopropylated surface by means of the azido-transfer reaction. Subsequently, D-amino acid adhesion peptides could be immobilized on the surface by use of Cu(I)-catalyzed click chemistry. This enabled the study of cell adhesion to the metal surface. In contrast to unmodified surfaces, the peptide-modified surfaces were able to maintain cell adhesion during significant flow velocities ...Continue Reading

References

Jul 30, 2005·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Bo W LaursenNiels Harrit
Dec 13, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·John A Howarter, Jeffrey P Youngblood
Feb 15, 2007·Analytical Chemistry·Shaurya PrakashMark A Shannon
Aug 14, 2008·Chemical Reviews·Morten Meldal, Christian Wenzel Tornøe
Apr 29, 2010·Chemical Society Reviews·Claudia HaenschUlrich S Schubert
Sep 20, 2011·Chembiochem : a European Journal of Chemical Biology·Morten MeldalGrith Hagel
Jun 22, 2012·Accounts of Chemical Research·Scott A Jewett, Albena Ivanisevic
Sep 26, 2013·Nanoscale·Katrine R RostgaardKaren L Martinez
Feb 19, 2014·The Journal of Physical Chemistry. C, Nanomaterials and Interfaces·Gilad ZornMingdi Yan

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Citations

Nov 26, 2015·Chemistry : a European Journal·Surendra VuttiMorten Meldal
May 29, 2016·Colloids and Surfaces. B, Biointerfaces·Fahimeh Shamsi
Oct 23, 2016·Dental Materials : Official Publication of the Academy of Dental Materials·Han Byul SongChristopher N Bowman
Sep 2, 2016·Journal of Peptide Science : an Official Publication of the European Peptide Society·Sergey NikitinFrederik Diness
Sep 17, 2016·Biological Chemistry·Mareen Pagel, Annette G Beck-Sickinger
Jun 9, 2021·Chemical Society Reviews·Limin WangStephanie Hoeppener
Oct 9, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Serena RigoCornelia G Palivan
Jul 20, 2019·ACS Applied Materials & Interfaces·William J BrittainJürgen Rühe

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