PMID: 12752453May 20, 2003Paper

Covalent and three-dimensional structure of the cyclodextrinase from Flavobacterium sp. no. 92

European Journal of Biochemistry
Hanna B FritzscheGeorg E Schulz

Abstract

Starting with oligopeptide sequences and using PCR, the gene of the cyclodextrinase from Flavobacterium sp. no. 92 was derived from the genomic DNA. The gene was sequenced and expressed in Escherichia coli; the gene product was purified and crystallized. An X-ray diffraction analysis using seleno-methionines with multiwavelength anomalous diffraction techniques yielded the refined 3D structure at 2.1 A resolution. The enzyme hydrolyzes alpha(1,4)-glycosidic bonds of cyclodextrins and linear malto-oligosaccharides. It belongs to the glycosylhydrolase family no. 13 and has a chain fold similar to that of alpha-amylases, cyclodextrin glycosyltransferases, and other cyclodextrinases. In contrast with most family members but in agreement with other cyclodextrinases, the enzyme contains an additional characteristic N-terminal domain of about 100 residues. This domain participates in the formation of a putative D2-symmetric tetramer but not in cyclodextrin binding at the active center as observed with the other cyclodextrinases. Moreover, the domain is located at a position quite different from that of the other cyclodextrinases. Whether oligomerization facilitates the cyclodextrin deformation required for hydrolysis is discussed.

References

Feb 20, 1991·Journal of Molecular Biology·C Klein, G E Schulz
Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Jan 1, 1981·Bioscience Reports·F Sanger
Mar 1, 1984·Journal of Biochemistry·Y MatsuuraM Kakudo
Dec 11, 1981·Science·F Sanger
May 27, 1994·Journal of Molecular Biology·A KadziolaR Haser
Jun 1, 1996·The Biochemical Journal·B Henrissat, A Bairoch
Oct 1, 1996·Journal of Pharmaceutical Sciences·T Loftsson, M E Brewster
Aug 28, 1998·Journal of Molecular Biology·Y KatsuyaY Matsuura
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Sep 3, 1999·The Journal of Biological Chemistry·J S KimB H Oh
Oct 6, 1999·Current Opinion in Structural Biology·I Usón, G M Sheldrick
Jan 3, 2001·Acta Crystallographica. Section D, Biological Crystallography·M D WinnG N Murshudov
Mar 21, 2001·Biochimica Et Biophysica Acta·E A MacGregorB Svensson
Mar 28, 2002·Applied and Environmental Microbiology·Hiroshi KamasakaTakashi Kuriki
Mar 30, 2002·The Journal of Biological Chemistry·Hee-Seob LeeKwan-Hwa Park
Dec 21, 2002·European Journal of Biochemistry·Hans LeemhuisLubbert Dijkhuizen
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Jul 1, 1997·Acta Crystallographica. Section D, Biological Crystallography·A PerrakisV S Lamzin
Jan 1, 1997·Methods in Enzymology·E A Merritt, D J Bacon
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

❮ Previous
Next ❯

Citations

May 21, 2013·Enzyme and Microbial Technology·Sook-Chen MokMaqsudul Alam
Aug 9, 2011·Bioscience, Biotechnology, and Biochemistry·Momoko KobayashiAtsuo Kimura
Jan 15, 2014·Applied Microbiology and Biotechnology·Deepika Mehta, T Satyanarayana
Nov 19, 2008·Journal of Molecular Biology·Stefan Buedenbender, Georg E Schulz
Feb 18, 2015·Archaea : an International Microbiological Journal·Ying SunJinliang Liu
May 4, 2016·Cellular and Molecular Life Sciences : CMLS·Matthew H FoleyNicole M Koropatkin
Dec 3, 2016·Microbiology·Andrea Kuchtová, Štefan Janeček
Mar 14, 2017·Scientific Reports·Fean D SarianMarc J E C van der Maarel
Jan 24, 2007·Biotechnology Journal·Manuel FerrerPeter N Golyshin
Sep 20, 2019·Biotechnology Advances·Štefan JanečekBirte Svensson
Aug 28, 2018·Protein Expression and Purification·Fabiane Cristina Dos Santos, Ione Parra Barbosa-Tessmann
Jan 31, 2012·International Journal of Biological Macromolecules·Tzu-Ting ChuangLong-Liu Lin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.