PMID: 7541400Jul 1, 1995Paper

Covalent association of beta-1,3-glucan with beta-1,6-glucosylated mannoproteins in cell walls of Candida albicans

Journal of Bacteriology
J C KapteynF M Klis

Abstract

Yeast and hyphal walls of Candida albicans were extracted with sodium dodecyl sulfate (SDS). Some of the extracted proteins reacted with a specific beta-1,6-glucan antiserum but not with a beta-1,3-glucan antiserum. They lost their beta-1,6-glucan epitope after treatment with ice-cold aqueous hydrofluoric acid, suggesting that beta-1,6-glucan was linked to the protein through a phosphodiester bridge. When yeast and hyphal walls extracted with SDS were subsequently extracted with a pure beta-1,3-glucanase, several mannoproteins that were recognized by both the beta-1,6-glucan antiserum and the beta-1,3-glucan antiserum were released. Both epitopes were sensitive to aqueous hydrofluoric acid treatment, suggesting that beta-1,3-glucan and beta-1,6-glucan are linked to proteins by phosphodiester linkages. The possible role of beta-glucans in the retention of cell wall proteins is discussed.

Citations

Mar 1, 1997·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·Y Fukazawa, K Kagaya
Mar 12, 2003·Medical Mycology·N KondoriI Mattsby-Baltzer
Oct 11, 2014·Folia Microbiologica·Lucila Ortiz-Castellanos, José Ruiz-Herrera
May 5, 2006·Molecular Biology of the Cell·Gertien J SmitsFrans M Klis
Aug 3, 2016·Cold Spring Harbor Protocols·Hiroki OkadaYoshikazu Ohya
Feb 15, 2000·Molecular Microbiology·S Shahinian, H Bussey
Oct 25, 1996·The Journal of Biological Chemistry·R P HartlandJ P Latgé
May 10, 2000·FEMS Microbiology Letters·R KandasamyW L Chaffin
Mar 8, 2003·Molecular Microbiology·Jonathan D CroweNuala A Booth
Mar 13, 2009·The Journal of Biological Chemistry·Vishukumar AimaniandaJean-Paul Latgé
May 15, 1995·FEMS Microbiology Letters·J C KapteynF M Klis
Nov 20, 2002·Infection and Immunity·Donatella PietrellaAnna Vecchiarelli
Aug 6, 1999·FEMS Immunology and Medical Microbiology·M UchiyamaT Yadomae
Jun 24, 2004·Infection and Immunity·Yoshiyuki AdachiNaohito Ohno
Sep 6, 2008·Microbiology and Molecular Biology Reviews : MMBR·W LaJean Chaffin
Sep 6, 2002·Microbiology·Muriel BernardJean-Paul Latgé
Jul 31, 1998·Journal of Bacteriology·P N Lipke, R Ovalle
May 5, 2021·Antonie van Leeuwenhoek·Maise Gomes QueirozMarcos Antonio de Morais Junior
Aug 25, 1999·Current Opinion in Microbiology·G J SmitsF M Klis

❮ Previous
Next ❯

Related Concepts

Related Feeds

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.