Covalent attachment and hybridization of DNA oligonucleotides on patterned single-walled carbon nanotube films

Langmuir : the ACS Journal of Surfaces and Colloids
Dae-Hwan JungHee-Tae Jung

Abstract

DNA oligonucleotides were covalently immobilized to prepatterned single-walled carbon nanotube (SWNT) multilayer films by amidation. SWNT multilayer films were constructed via consecutive condensation reactions creating stacks of functionalized SWNT layers linked together by 4,4'-oxydianiline. Aminated- or carboxylated-DNA oligonucleotides were covalently immobilized to the respective carboxylated or aminated SWNT multilayer films through amide bond formation using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride. UV-vis-NIR spectroscopic analysis indicated that the SWNT film surface density increased uniformly according to the number of reaction cycles. Scanning electron microscopy and contact angle measurements of the SWNT multilayer film revealed a uniform coverage over the substrate surface. The covalent attachment of DNA oligonucleotides to the SWNT multilayer films and their subsequent hybridization with complementary oligonucleotides were verified using X-ray photoelectron spectroscopy and fluorescence-based measurements. This is the first report demonstrating that DNA oligonucleotides can be covalently attached to immobilized SWNT multilayer films. The anchored DNA oligonucleotides were shown to exhibit excel...Continue Reading

References

Apr 1, 1994·Analytical Biochemistry·D Sehgal, I K Vijay
Jun 5, 1998·Science·J LiuR E Smalley
Nov 20, 1998·Journal of Colloid and Interface Science·S ShibuichiK Tsujii
Jan 29, 2000·Science·J KongH Dai
Feb 26, 2000·Science·J S MartinezA Butler
Jul 13, 2000·Journal of Combinatorial Chemistry·J P PelloisX Gao
May 9, 2001·The Analyst·B A CavicM Thompson
Feb 19, 2002·Chemical Reviews·P. M. Ajayan
Jun 20, 2002·Journal of the American Chemical Society·Sheila R Nicewarner PeñaChristine D Keating
Jul 6, 2002·Science·Kinneret KerenErez Braun
Aug 6, 2002·Science·Ray H BaughmanWalt A de Heer
Nov 22, 2002·Proceedings of the National Academy of Sciences of the United States of America·H-T JungJ A Zasadzinski
Feb 20, 2004·Chemical Communications : Chem Comm·Dae-Hwan JungHee-Tae Jung

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Citations

Feb 9, 2010·Journal of the American Chemical Society·Pradip BagRobert C Haddon
Nov 6, 2013·Analytical Biochemistry·Yanyang LiuKun-Lin Yang
Apr 1, 2014·Colloids and Surfaces. B, Biointerfaces·Arzum Erdem, Gulsah Congur
Jan 1, 2008·Nanoscale Research Letters·Yanyan LiuFangfang Xu
Jul 23, 2013·Methods : a Companion to Methods in Enzymology·Gustavo A Zelada-GuillénJordi Riu
Nov 28, 2014·Sensors·Satish Balasaheb NimseTaisun Kim
Mar 4, 2014·ACS Applied Materials & Interfaces·Yanan ChenAlexander Star
Aug 18, 2009·Journal of Immunological Methods·Phuong Dinh TamMai Anh Tuan
Jun 20, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Zhuoqi ZhangJian R Lu
Jul 6, 2005·Chembiochem : a European Journal of Chemical Biology·Hye Ryung ByonHee Cheul Choi
Feb 8, 2013·Small·Ali Yasin Sonay, Mustafa Culha
Dec 12, 2013·Biosensors & Bioelectronics·Rafael HernándezJordi Riu
Sep 29, 2012·Biosensors & Bioelectronics·Gustavo A Zelada-GuillénF Xavier Rius
Jun 20, 2013·Colloids and Surfaces. B, Biointerfaces·Murilo F CabralSergio A S Machado
Dec 20, 2013·Chemical Society Reviews·Vinalia TjongAshutosh Chilkoti
Jul 19, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Jie ChenDavid W Schmidtke
Aug 24, 2013·Nanotechnology·Taekyeong KimSeunghun Hong
Jul 17, 2018·Scanning·Kazuo Umemura, Shizuma Sato
Aug 7, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Byung-Seon KongHee-Tae Jung
Dec 22, 2007·Chemical Reviews·Audrey SassolasLoïc J Blum
Oct 22, 2010·Analytical Chemistry·Gustavo A Zelada-GuillénF Xavier Rius
Feb 9, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Mackenzie G WilliamsAndrew Teplyakov
Nov 10, 2018·Bioconjugate Chemistry·Jacopo MovilliJurriaan Huskens
Mar 9, 2006·Chemical Reviews·Dimitrios TasisMaurizio Prato

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