PMID: 6163457Jan 8, 1981Paper

Covalent attachment of immunoglobulins to liposomes via glycosphingolipids

Biochimica Et Biophysica Acta
T D HeathD Papahadjopoulos

Abstract

We describe a method for covalent binding of proteins to large unilamellar liposomes which involves the periodate oxidation of glycosphingolipids in the vesicle membrane. Proteins such as IgG and F(ab')2 may then be attached to the aldehyde groups on the glycolipid by Schiff-base formation at pH 9.5 and reduction with NaBH4, or by reductive amination with NaBH3CN at pH 8.4. Exposure of the vesicles to periodate, protein coupling and separation from unbound protein by a novel method of flotation is discontinuous dextran gradients does not release the vesicle contents when performed at pH 8.4. Studies on the oxidation of neutral glycolipid-containing vesicles, and on the oxidation of encapsulated glycerol 1-phosphate show that periodate influx into neutral vesicles during a 4 h exposure is appreciable at pH 5.5 but not at pH 8.4. Under optimal conditions, approx. 20% of the protein may be coupled to vesicles, and a ratio of 100--200 microgram of protein/mumol of lipid is readily achieved. This method will be of great importance for the antibody-mediated targeting of vesicles to cells.

References

Oct 19, 1979·Biochimica Et Biophysica Acta·F OlsonD Papahadjopoulos
Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·F Szoka, D Papahadjopoulos
Aug 28, 1979·Biochemical and Biophysical Research Communications·V P TorchilinE Haber
Nov 15, 1979·Biochimica Et Biophysica Acta·B A MacherP H Fishman
Dec 14, 1978·Biochemical and Biophysical Research Communications·V P TorchilinV N Smirnov
Dec 14, 1976·Biochimica Et Biophysica Acta·D A TyrrellB E Ryman
May 12, 1975·Analytical Biochemistry·G Rajagopal, S Ramakrishnan
Jun 20, 1980·Biochimica Et Biophysica Acta·T D HeathA J Davies
Feb 1, 1974·The Journal of Clinical Investigation·G WeissmannE C Franklin
Jan 1, 1956·Methods of Biochemical Analysis·J R DYER
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Aug 1, 1963·The Biochemical Journal·R H PAIN

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Citations

Dec 16, 1983·Pharmaceutisch Weekblad. Scientific Edition·P A Toonen, D J Crommelin
Mar 1, 1993·Applied Biochemistry and Biotechnology·Y SohmaE Sada
Jun 1, 1988·Biochemical Pharmacology·P A PeetersD J Crommelin
Sep 1, 1993·Chemistry and Physics of Lipids·J A BouwstraH Talsma
Nov 10, 1994·Journal of Immunological Methods·K TomiokaS Katoh
Jan 1, 1984·Pharmacology & Therapeutics·J N Weinstein, L D Leserman
Nov 1, 1985·Gan to kagaku ryoho. Cancer & chemotherapy·H Sezaki
May 26, 1997·Journal of Immunological Methods·H A RongenW P van Bennekom
Sep 1, 2000·Advanced Drug Delivery Reviews·N YamazakiH J Gabius
Mar 26, 2002·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·R T GomesT V Freitas
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·J HuwylerW M Pardridge
Jan 1, 1990·Annals of the New York Academy of Sciences·M KishimuraH Taniguchi
Nov 18, 2011·The Journal of the Acoustical Society of America·Jonathan A KopechekChristy K Holland
Dec 18, 2001·Analytical Biochemistry·Melvin E KlegermanDavid D McPherson
Dec 29, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yvonne M te WelscherWayne I Lencer
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·T D HeathD Papahadjopoulos
Jan 25, 2011·International Journal of Pharmaceutics·Yao-Da Dong, Ben J Boyd
Jan 1, 1985·Annals of the New York Academy of Sciences·D PapahadjopoulosK Matthay
Jan 1, 1987·Annals of the New York Academy of Sciences·J M ShawL B Schook
Sep 21, 2005·International Journal of Pharmaceutics·Rong Deng, Joseph P Balthasar
Nov 26, 2003·Biochimica Et Biophysica Acta·Vivek S PurohitSathyamangalam V Balasubramanian
Nov 26, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Arehalli S ManjappaRayasa S Ramachandra Murthy
Feb 1, 1989·Analytical Biochemistry·A L PlantR A Durst
Apr 1, 1982·Journal of Pharmaceutical Sciences·K J WidderB Sears
Nov 1, 1983·Analytical Biochemistry·S Carpenter-Green, L Huang
Jul 1, 1984·Scandinavian Journal of Immunology·L BjörckR Sundler
Dec 15, 1992·European Journal of Biochemistry·G Zardeneta, P M Horowitz
Jan 1, 1985·Annals of the New York Academy of Sciences·S L Regen
Apr 15, 1993·Biotechnology and Bioengineering·S KatohH Fukuda
Jun 1, 1991·Immunological Investigations·A K Sarkar, M K Das

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