Covalent binding of [14C]thiourea to protein in lungs from endotoxin-treated rats

Toxicology and Applied Pharmacology
M A Hollinger

Abstract

Administration of thiourea to mature male rats at a dosage of 3.5 mg/kg (ip) produced marked pleural effusion by 2 hr (3-4 ml). Pretreatment with bacterial lipopolysaccharide (endotoxin) significantly reduced this pleural effusion (less than 0.5 ml). Despite this unequivocal effect, there was no corresponding reduction in the covalent binding of [14C]thiourea to lung protein. These data indicate that the protective effect of endotoxin against the initial stages of thiourea pneumotoxicity does not involve a reduction in binding of the [14C]thiourea or a metabolite to lung protein. However, alterations in low levels of binding to specific cell types or particular protein(s) relative to covalent binding cannot be ruled out.

References

Feb 1, 1978·The Journal of Clinical Investigation·L FrankR J Roberts

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