Covalent binding of C3b to tetanus toxin: influence on uptake/internalization of antigen by antigen-specific and non-specific B cells

Immunology
M B VilliersM Colomb

Abstract

Antigen opsonization by the C3b fragment of complement is a significant event in the modulation of cell-mediated immune response, but its mechanism is still largely unknown. The structural characteristics of C3b allow it to act as a bifunctional ligand between antigen and cells via their membrane C3b receptors. It was thus of interest to study the influence of the covalent link between C3b and antigen on the fixation and internalization of this antigen by antigen-presenting cells. Tetanus toxin (TT) was used as antigen, either free or covalently linked to C3b (TT-C3b). The antigen-presenting cells were TT-specific (4.2) or non-specific (BL15) Epstein-Barr virus (EBV)-transformed B cells. C3b was found to play an important role in antigen fixation and internalization by both antigen-specific and antigen non-specific cells. Covalent binding of C3b on TT (1) permitted fixation and internalization of this antigen by non-specific cells via their complement receptors; (2) enhanced antigen fixation and resulted in cross-linking between membrane immunoglobulins and complement receptors on antigen-specific cells. The consequences of covalent C3b binding to TT were analysed using antigen-specific and antigen-nonspecific cells. In both ca...Continue Reading

References

Sep 1, 1991·Immunology Today·A ErdeiJ Gergely
Jul 1, 1991·Molecular Immunology·C D BarroM G Colomb
Nov 1, 1991·Scandinavian Journal of Immunology·M B VilliersM G Colomb
Dec 1, 1989·The Journal of Experimental Medicine·J M PesandoB E McMaster
Apr 11, 1985·Nature·A Lanzavecchia
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·A Dautry-VarsatH F Lodish

❮ Previous
Next ❯

Citations

Apr 4, 2002·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·R MeerwaldtM O Hoekstra
May 16, 2000·Immunology·C H NielsenR G Leslie
Jan 8, 2010·Nucleic Acids Research·Prabhat K MallikHua Shi
Nov 25, 2003·Cell Stress & Chaperones·Olivier Preynat-SeauveChristian Villiers
Aug 17, 2011·International Journal of Environmental Research and Public Health·David BittnerCarlo R Largiadèr
Jul 1, 2008·Molecular Immunology·Christian L VilliersMarie-Bernadette Villiers
Nov 25, 2005·Scandinavian Journal of Immunology·F HjelmB Heyman
Dec 21, 2005·Immunology Letters·Andrew Getahun, Birgitta Heyman
Aug 20, 2005·Journal of Immunological Methods·Denise V BarraultAndrew M Knight
Jun 14, 2005·Fish & Shellfish Immunology·H BoshraJ O Sunyer
Jul 9, 2014·Molecular Immunology·Anna SörmanBirgitta Heyman
Apr 24, 2009·Vaccine·Panisadee AvirutnanMichael S Diamond
Dec 25, 2010·Biomaterials·Susan N ThomasJeffrey A Hubbell
Nov 18, 2014·PloS One·Marie Klinge BrimnesClaus Henrik Nielsen
Jan 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Antje HeitHermann Wagner
Mar 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Laure A Perrin-CoconPatrice N Marche
Jul 5, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hidekazu ShirotaGen Tamura
Mar 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mihály JózsiAnna Erdei

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibodies: Complement Activation

The complement system can be activated by antigen-associated antibody. In the classical pathway of complement activation, C1q, C4b, and C3b are all able to bind to the Fc portion of IgG or IgM. Find the latest research on antibodies and complement activation here.

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.

Antibody-Dependent Cell Cytotoxicity

Antibody-dependent cellular toxicity refers to the lysis of a target cell by a non-sensitized effector cell of the immune system as a result of antibodies binding to the target cell membrane and engaging the Fc receptors on the immune effector cells. Find the latest research on antibody-dependent cellular toxicity here.

Alternative Complement Pathway

The Alternative Complement Pathway is part of the innate immune system, and activation generates membrane attack complexes that kill pathogenic cells. Discover the latest research on the Alternative Complement Pathway.