Covalent immobilization of hirudin improves the haemocompatibility of polylactide-polyglycolide in vitro

Biomaterials
B SeifertT Groth

Abstract

A biodegradable polymer, poly(D,L-lactide-co-glycolide) RESOMER RG756, was modified by surface immobilization of recombinant hirudin (r-Hir) with glutaraldehyde as coupling reagent to improve the blood contacting properties of the polymer. The activity of immobilized hirudin on the polymer was estimated by a chromogenic assay to about 2.5 ATU r-Hir cm-2. The improvement of the haemocompatibility of the modified RG756 was evaluated in terms of platelet adhesion/activation, whole blood clotting times and clot formation rate. Fluorescence microscopy revealed that surface modification with r-Hir resulted in decreased platelet adhesion and activation. An ELISA for P-selectin, a marker of platelet activation, was used to confirm this result. Clotting time experiments demonstrated significantly prolonged non-activated partial thromboplastin times, and a decreased clot formation rate of whole blood in contact with r-Hir modified RG756 compared with the plain polymer. Comparison of immobilized r-Hir with bound heparin yielded equivalent improvement of blood-contacting properties of the investigated polymers. These in vitro investigations indicate that the immobilization of r-Hir on RG756 is a useful method to improve the blood contactin...Continue Reading

References

Oct 1, 1992·Journal of Comparative Pathology·P A Gentry
Jan 1, 1991·Haemostasis·F MarkwardtE Bucha
May 1, 1972·Journal of Biomedical Materials Research·Y Imai, Y Nose
Jan 1, 1995·Journal of Biomaterials Science. Polymer Edition·B SeifertP Romaniuk
Jul 1, 1994·Coronary Artery Disease·H M van BeusekomW J van der Giessen
Aug 1, 1994·Biomaterials·J M CourtneyC D Forbes
Mar 1, 1993·AJR. American Journal of Roentgenology·J C Palmaz

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Citations

Aug 11, 2001·Journal of Biomedical Materials Research·M A Ruegsegger, R E Marchant
Sep 1, 2005·Analytical and Bioanalytical Chemistry·Vasilis G GavalasLeonidas G Bachas
Jun 21, 2005·Journal of Materials Science. Materials in Medicine·Qinggang TanJia-Cong Shen
Mar 12, 2004·Thrombosis Research·McDonald K Horne, Kimberly J Brokaw
Mar 16, 2001·International Journal of Pharmaceutics·M J MontisciG Ponchel
Mar 7, 2002·Artificial Organs·B SeifertG von Sengbusch
Nov 28, 2013·Journal of Materials Science. Materials in Medicine·Amaliris RuizKristyn S Masters
Jun 7, 2011·Annual Review of Biomedical Engineering·Song Li, Jeffrey J D Henry
Apr 7, 2011·Tissue Engineering. Part a·John D StroncekWilliam M Reichert
Dec 20, 2011·Advanced Functional Materials·Zheng QuElliot L Chaikof
Nov 13, 2003·Journal of Biomedical Materials Research. Part a·Eeva-Maija HietalaRiitta Lassila
Mar 7, 2006·Expert Review of Medical Devices·Gemma ConnAlexander M Seifalian
Aug 8, 2007·Journal of Vascular Surgery·Sumanas W Jordan, Elliot L Chaikof
Dec 10, 2013·Acta Biomaterialia·Sidónio C FreitasM Cristina L Martins
Mar 19, 2020·Artificial Organs·Paul S Malchesky
Sep 21, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xiaoli LiuJohn L Brash

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