Covalent immobilization of invertase on polyurethane, plast-film and ferromagnetic Dacron

Bioresource Technology
P G CadenaM C B Pimentel

Abstract

Invertase was covalently immobilized on polyurethane (PU), inox plate covered with plast-film layer and ferromagnetic azide-Dacron. The immobilization processes, physico-chemical parameters and a model for coupling reactions were studied. The preliminary studies for selection of the support showed that the best activity was obtained for PU treated with HCl, polyethylenimine and glutaraldehyde (156.7+/-4.9 U/g support). All plast-film-invertase derivatives did not show activity and the Dacron-invertase derivative showed an activity of 105.39 U/g support. The invertase immobilized in presence of substrate (10% w/v sucrose) was the most efficient (832.74+/-1.48 U/g support). The optimal pH was shifted from 4.5 (free enzyme) to 5.0 (immobilized derivative) and optimal temperature was not affected. Activation energy values of free enzyme, Dacron-invertase and PU-invertase were 32.4+/-0.34 kJ/mol, 33.4+/-0.36 kJ/mol and 44.0+/-0.67 kJ/mol, respectively. The PU-invertase could be used over 2 months without considerable activity loss (68.5% activity retention) and retained 12.6% (287.97+/-27.9U/g support) of the activity after five cycles.

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Citations

Sep 13, 2013·Applied Biochemistry and Biotechnology·Zehra OlcerAziz Tanriseven
Sep 30, 2010·Bioresource Technology·Pabyton G CadenaMaria C B Pimentel
Sep 28, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jose J Virgen-OrtízRoberto Fernandez-Lafuente
Jul 6, 2021·Applied Microbiology and Biotechnology·B I S DaminA Dettmer
Aug 14, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·S L HirshD R McKenzie
Dec 2, 2021·Journal of Applied Microbiology·Letícia Mara RasboldAlexandre Maller

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