Covalent Linkage of One-Dimensional DNA Arrays Bonded by Paranemic Cohesion

ACS Nano
Yoel P OhayonNadrian C Seeman

Abstract

The construction of DNA nanostructures from branched DNA motifs, or tiles, typically relies on the use of sticky-ended cohesion, owing to the specificity and programmability of DNA sequences. The stability of such constructs when unligated is restricted to a specific range of temperatures, owing to the disruption of base pairing at elevated temperatures. Paranemic (PX) cohesion was developed as an alternative to sticky ends for the cohesion of large topologically closed species that could be purified reliably on denaturing gels. However, PX cohesion is also of limited stability. In this work, we added sticky-ended interactions to PX-cohesive complexes to create interlocked complexes by functionalizing the sticky ends with psoralen, which can form cross-links between the two strands of a double helix. We were able to reinforce the stability of the constructs by creating covalent linkages between the 3'-ends and 5'-ends of the sticky ends; the sticky ends were added to double crossover domains via 3'-3' and 5'-5' linkages. Catenated arrays were obtained either by enzymatic ligation or by UV cross-linking. We have constructed finite-length one-dimensional arrays linked by interlocking loops and have positioned streptavidin-gold pa...Continue Reading

References

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Citations

Mar 2, 2016·Chembiochem : a European Journal of Chemical Biology·Arun Richard ChandrasekaranXing Wang
Nov 26, 2015·Chembiochem : a European Journal of Chemical Biology·Christos PanagiotidisGünter von Kiedrowski
Apr 5, 2016·Small·Arun Richard ChandrasekaranHari K K Subramanian
Dec 8, 2016·Organic & Biomolecular Chemistry·Weili ShenNadrian C Seeman
Dec 23, 2017·Nucleic Acids Research·Laurent AzémaJean-Jacques Toulmé
Jul 13, 2016·Organic & Biomolecular Chemistry·Weili ShenChengde Mao
Jun 6, 2019·Beilstein Journal of Organic Chemistry·Shahien ShahsavariShiyue Fang
Jan 1, 2016·Nanobiomedicine·Arun Richard Chandrasekaran
Nov 6, 2018·Nature Communications·Xiaodong QiHao Yan
Feb 9, 2016·Nanoscale·Arun Richard Chandrasekaran
Dec 13, 2017·Nanotechnology·P Lourdu Xavier, Arun Richard Chandrasekaran
Mar 27, 2020·Journal of the American Chemical Society·Arun Richard ChandrasekaranKen Halvorsen
Jun 19, 2018·Chemical Reviews·Xing WangNadrian C Seeman
Apr 7, 2020·ACS Applied Materials & Interfaces·Xueting WangYin Liu
Dec 9, 2021·Biopolymers·Jordi F Keijzer, Bauke Albada

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