PMID: 2496661May 15, 1989Paper

Covalent structure of collagen: amino acid sequence of three cyanogen bromide-derived peptides from human alpha 1(V) collagen chain

Archives of Biochemistry and Biophysics
J M Seyer, A H Kang

Abstract

Type V collagen was prepared from human amnionic/chorionic membranes and separated into alpha 1(V) and alpha 2(V) polypeptide chains. The alpha 1(V) chain was digested with cyanogen bromide and nine peptides were obtained and purified. Three of the peptides, alpha 1(V)CB1, CB4, and CB7 having molecular weights of 5000, 8000, and 6000, respectively, were further analyzed by amino acid sequence analysis and thermolytic or tryptic digestions. CB1 contained 54 amino acids and identification of its complete sequence was aided by thermolysin digestion and isolation of two peptides, Th1 and Th2. CB4 contained 81 amino acids and sequence analysis of intact CB4 and five tryptic peptides provided us with its complete amino acid sequence. The peptide CB7 contained 67 amino acids and was cleaved into four tryptic peptides that were used for complete sequence analysis. The above results represent the first available covalent structure information on the alpha 1(V) collagen chain. These data enabled us to establish the location of these peptides within the helical structure of other collagen chains. CB4 was homologous to residues 66-145 in the collagen chain while CB1 represented residues 146-200 and CB7 was homologous with residues 201-269....Continue Reading

References

Mar 1, 1979·FEBS Letters·H von der Mark, H von der Mark
Apr 27, 1979·Biochemical and Biophysical Research Communications·R E Burgeson, D W Hollister
Jul 27, 1979·Biochemical and Biophysical Research Communications·Z DeylM Adam
Feb 28, 1978·Biochemical and Biophysical Research Communications·R A BrownJ B Weiss
Aug 23, 1976·Biochemical and Biophysical Research Communications·E ChungE J Miller
Mar 1, 1988·The Journal of Cell Biology·D E BirkT F Linsenmayer
Jan 1, 1985·Annals of the New York Academy of Sciences·J H FesslerL I Fessler
Dec 19, 1973·Biochemical and Biophysical Research Communications·C L ZimmermanE Appella
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J M FitchT F Linsenmayer
Oct 1, 1983·Experimental and Molecular Pathology·T M HeringJ M Anderson
Mar 1, 1984·Collagen and Related Research·S AyadL Holt
Feb 1, 1981·Collagen and Related Research·J M FoidartG R Martin
Sep 15, 1981·Biochemistry·C A Reese, R Mayne
Feb 1, 1982·The Journal of Cell Biology·A Martinez-HernandezE J Miller

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