Covalent Whey Protein-Rosmarinic Acid Interactions: A Comparison of Alkaline and Enzymatic Modifications on Physicochemical, Antioxidative, and Antibacterial Properties

Journal of Food Science
Mostafa AliKarin Schwarz

Abstract

The covalent interactions between whey protein isolate (WPI) and rosmarinic acid (RosA) at two different conditions, alkaline (pH 9) and enzymatic (in the presence of tyrosinase, PPO), at room temperature with free atmospheric air were studied. The conjugates formed between WPI and RosA were characterized in terms of their physicochemical and functional properties. The changes in protein structure were analyzed by intrinsic fluorescence and binding of 8-anilino-1-naphthalenesulfonic acid. The findings show that the covalent interactions caused a decrease in free amino and thiol groups and tryptophan content at both conditions. The decrease at enzymatic conditions was lower than at alkaline conditions. In addition, modified WPI at alkaline conditions exhibited higher antioxidative capacity compared to the modification at enzymatic conditions. However, WPI modified at enzymatic condition showed mild antimicrobial activity against Staphylococcus aureus LMG 10147 and MU50 compared to WPI modified at alkaline conditions and unmodified WPI (control). The modified WPI can be used as multifunctional ingredient into various food products with an additional health promoting effect of the bound phenolic compounds.

References

Jan 1, 1978·Advances in Food Research·K Hofmann, R Hamm
Nov 1, 1976·Journal of Agricultural and Food Chemistry·J Adler-Nissen
Jun 25, 1999·Free Radical Biology & Medicine·R ReC Rice-Evans
Feb 26, 2000·Journal of Agricultural and Food Chemistry·N Alizadeh-Pasdar, E C Li-Chan
May 31, 2001·Die Nahrung·H M RawelU C Hohl
Apr 13, 2004·Protein Science : a Publication of the Protein Society·Thomas CroguennecSaïd Bouhallab
Jul 22, 2004·Journal of Agricultural and Food Chemistry·Sascha RohnJürgen Kroll
May 12, 2005·Journal of Agricultural and Food Chemistry·Harshadrai M RawelJürgen Kroll
Jun 1, 2005·Biophysical Journal·Roger ParkerStephen G Ring
Oct 18, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Pinaki R MajhiPaul L Dubin
Aug 8, 2007·Insect Biochemistry and Molecular Biology·Picheng ZhaoHaobo Jiang
Oct 18, 2008·Mutation Research·Michelle Andrade FurtadoDenise Crispim Tavares
Nov 7, 2009·The American Journal of Pathology·Tsuyoshi HamaguchiMasahito Yamada
Dec 25, 2010·Journal of Dairy Science·L ChanetonL E Bussmann
Jun 25, 2013·Journal of Agricultural and Food Chemistry·Mostafa AliHarshadrai Rawel
Mar 13, 2014·Journal of Agricultural and Food Chemistry·Juan YouJianping Wu
Dec 10, 2014·International Journal of Molecular Sciences·Amar B T Ghisaidoobe, Sang J Chung
Jan 20, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Verena BongartzAndreas Schieber
Jan 11, 2017·Journal of Food Science·Yanyun Cao, Youling L Xiong
Jan 10, 2018·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Dimitris KarefyllakisConstantinos V Nikiforidis

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Citations

Jul 28, 2020·Journal of Biochemical and Molecular Toxicology·Yang YaoYong Wang

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