PMID: 2505251Sep 1, 1989Paper

Covalently bound phosphate residues in bovine milk xanthine oxidase and in glucose oxidase from Aspergillus niger: a reevaluation

Proceedings of the National Academy of Sciences of the United States of America
J L JohnsonK V Rajagopalan

Abstract

The reported presence of covalently bound phosphate residues in flavoproteins has significant implications with regard to the catalytic mechanisms and structural stability of the specific enzymes themselves and in terms of general cellular metabolic regulation. These considerations have led to a reevaluation of the presence of covalently bound phosphorus in the flavoproteins xanthine oxidase (xanthine: oxygen oxidoreductase, EC 1.1.3.22) and glucose oxidase (beta-D-glucose: oxygen 1-oxidoreductase, EC 1.1.3.4). Milk xanthine oxidase purified by a procedure that includes anion-exchange chromatography is shown to contain three phosphate residues. All three are noncovalently associated with the protein, two with the FAD cofactor, and one with the molybdenum cofactor. Results of chemical analysis and 31P NMR spectroscopy indicate that enzyme purified by this method contains no phosphoserine residues. Xanthine oxidase preparations purified by chromatography on calcium phosphate gel in place of DEAE-Sephadex yielded higher phosphate-to-protein ratios, which could be reduced to the expected values by additional purification on a folate affinity column. Highly active, highly purified preparations of glucose oxidase are shown to contain...Continue Reading

References

Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D E Edmondson, T L James
Aug 1, 1975·Archives of Biochemistry and Biophysics·W R WaudK V Rajagopalan
Feb 17, 1986·European Journal of Biochemistry·J KlugkistC Veeger
May 1, 1973·Analytical Biochemistry·E J Faeder, L M Siegel

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Citations

Mar 12, 2008·Applied Microbiology and Biotechnology·Chun Ming WongXiao Dong Chen
Sep 5, 2002·Free Radical Biology & Medicine·Roger Harrison
May 13, 2009·Journal of Biotechnology·Karina J Paz-AlfaroJosé G Sampedro
Mar 8, 1994·Biochimica Et Biophysica Acta·R Hille
Apr 1, 1991·Archives of Biochemistry and Biophysics·D A Clare, J G Lecce
Aug 15, 2003·Preparative Biochemistry & Biotechnology·Hassan Y Aboul-EneinMagda Abd El-Aziz
Apr 11, 2017·Nature Chemical Biology·Carolyn E LubnerJohn W Peters
Mar 30, 2001·The Journal of Biological Chemistry·U S KayyaliP M Hassoun
Apr 15, 1991·Biotechnology and Bioengineering·G Fortier, D Bélanger
Jul 15, 1993·European Journal of Biochemistry·A Schieber, D E Edmondson
Mar 28, 1991·European Journal of Biochemistry·C SannerA Bacher
Nov 7, 1996·Chemical Reviews·Russ Hille

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