PMID: 8615032Apr 1, 1996Paper

Covalently crosslinked complexes of human immunodeficiency virus type 1 (HIV-1) gp120 and CD4 receptor elicit a neutralizing immune response that includes antibodies selective for primary virus isolates

Virology
Anthony DeVicoR Pal

Abstract

Specific conformational changes in the envelope glycoprotein gp120 of the human immunodeficiency virus type-1 (HIV-1) may be critical for eliciting a broadly neutralizing immune response against primary virus isolates. Since the interaction of gp120 with its receptor, CD4, induces conformational perturbations in both molecules, gp120-CD4 complexes should present unique immunogenic features that may include novel epitopes for broadly neutralizing antibodies. To test this hypothesis, we raised polyclonal antiserum against covalently crosslinked gp120-CD4 complexes in a goat and examined the ability of the anti-complex antibodies to neutralize primary and laboratory-adapted HIV-1 isolates. In cell-free neutralization assays with HIV-1MN, the antiserum demonstrated the ability to neutralize primary virus more effectively than the laboratory-adapted isolate. The neutralizing capacity of the anti-complex serum extended to primary isolates from distant genetic clades A, D, and E, although the degree of neutralization was found to vary among the clades. The neutralizing activity of the serum was composed of two components. The first component included anti-CD4 antibodies that recognized epitopes outside the gp120 binding site; the seco...Continue Reading

Citations

Apr 14, 2011·The Journal of Biological Chemistry·Grégoire MartinLoïc Martin
Dec 7, 2013·Retrovirology·Anna Roitburd-BermanJonathan M Gershoni
Aug 23, 2002·Proceedings of the National Academy of Sciences of the United States of America·Timothy FoutsRanajit Pal
Jan 31, 2002·AIDS·A M Schultz, J A Bradac
Mar 9, 2006·Current HIV/AIDS Reports·Isaac R Rodriguez-ChavezM Patricia D'Souza
Jul 11, 2006·Expert Review of Vaccines·Barton F Haynes, David C Montefiori
Sep 23, 2006·Expert Review of Vaccines·Barton F Haynes, David C Montefiori
May 20, 2016·Clinical and Vaccine Immunology : CVI·Jennifer A SchwartzTimothy R Fouts
Oct 10, 2009·The Journal of Physical Chemistry. B·Lin-Tai DaYun-Dong Wu

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