PMID: 9546177Apr 18, 1998Paper

COVASIAM: an image analysis method that allows detection of confluent microbial colonies and colonies of various sizes for automated counting

Applied and Environmental Microbiology
G CorkidiJ Nieto-Sotelo

Abstract

In this work we introduce the confluent and various sizes image analysis method (COVASIAM), an automated colony count technique that uses digital imaging technology for detection and separation of confluent microbial colonies and colonies of various sizes growing on petri dishes. The proposed method takes advantage of the optical properties of the surfaces of most microbial colonies. Colonies in the petri dish are epi-illuminated in order to direct the reflection of concentrated light coming from a halogen lamp towards an image-sensing device. In conjunction, a multilevel threshold algorithm is proposed for colony separation and counting. These procedures improved the quantification of colonies showing confluence or differences in size. We tested COVASIAM with a sample set of microorganisms that form colonies with contrasting physical properties: Saccharomyces cerevisiae, Aspergillus nidulans, Escherichia coli, Azotobacter vinelandii, Pseudomonas aeruginosa, and Rhizobium etli. These physical properties range from smooth to hairy, from bright to opaque, and from high to low convexities. COVASIAM estimated an average of 95.47% (sigma = 8.55%) of the manually counted colonies, while an automated method based on a single-threshold...Continue Reading

References

Jul 1, 1991·Genes & Development·M A StringerW E Timberlake
Jun 1, 1990·Science·Y Sanchez, S L Lindquist
Apr 1, 1984·Journal of Bacteriology·K D NoelM A Cevallos
Feb 1, 1995·International Journal of Bio-medical Computing·D P MukherjeeD D Majumder
Mar 1, 1995·American Journal of Clinical Pathology·D A Pickett, D F Welch
Feb 1, 1993·International Journal of Radiation Biology·I Spadinger, B Palcic
Aug 1, 1995·Applied and Environmental Microbiology·I G Wilson

❮ Previous
Next ❯

Citations

Feb 9, 2010·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Matthew L ClarkeJeeseong Hwang
Mar 26, 2003·FEBS Letters·Adriana Garay-ArroyoAlejandra A Covarrubias
Aug 31, 2010·Journal of Biomedical Optics·Euiwon BaeE Daniel Hirleman
Nov 27, 2001·Physics in Medicine and Biology·R BernardF Pernus
Mar 27, 2012·PloS One·Silvio D BruggerKathrin Mühlemann
Aug 30, 2012·Journal of Biological Engineering·Euiwon BaeJ Paul Robinson
Dec 7, 2018·PLoS Computational Biology·Hayden TronnoloneBenjamin J Binder
Jan 1, 2020·Computer Methods and Programs in Biomedicine·Paolo AndreiniFranco Scarselli

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society
Sergio VeraMiguel A Gonzalez Ballester
Applied Microbiology
D A Kékessy, J D Piguet
© 2022 Meta ULC. All rights reserved