COVID diagnostics by molecular methods: A systematic review of nucleic acid based testing systems.

Indian Journal of Medical Microbiology
Parijat DasMammen Chandy

Abstract

The selection of appropriate kit and PCR equipment for the detection of SARS CoV-2 is critically important in view of many options available in the diagnostic market. Since last year many molecular products are available for COVID-19 diagnostics., some of these diagnostics have become commercially available for healthcare workers and clinical laboratories. However, the diagnostic technologies have specific limitations and reported several false-positive and false-negative cases, especially during the early stages of kit development and use. The current article addresses these and other relevant questions important to the medical microbiologists running or aspiring to run COVID diagnostic services using PCR and related technologies. In this Systematic Review we follow Preferred Reporting Items for a Systematic Review and Meta-analysis of Diagnostic Test Accuracy Studies (PRISMA-DTA). A total of 258 citations retrieved, among those 77 peer reviewed articles was assessed for eligibility, and 181 studies were excluded. Based on inclusion criteria final data extraction was done. The question of diagnostic dilemma has also been addressed in view of discordant results between assays, inter-test variability, repeat testing requirements...Continue Reading

References

Jun 3, 2008·Methods in Molecular Biology·Haiyan Guan, Kaiping Yang
Apr 7, 2018·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Zhaozhong ZhuYousong Peng
Jan 17, 2019·Viruses·Zhiqi SongChuan Qin
Feb 1, 2020·The New England Journal of Medicine·Michelle L HolshueUNKNOWN Washington State 2019-nCoV Case Investigation Team
Feb 28, 2020·The Lancet Infectious Diseases·Yang PanQuanyi Wang
Apr 1, 2020·ACS Nano·Buddhisha UdugamaWarren C W Chan
Apr 5, 2020·Journal of Clinical Microbiology·Yi-Wei TangCharles W Stratton
Apr 29, 2020·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Bernard La ScolaDidier Raoult
Jun 4, 2020·Annals of Internal Medicine·Daniel P Oran, Eric J Topol
Aug 15, 2020·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Anika SinganayagamRobin Gopal
Oct 9, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Chanu RheeMichael Klompas
Apr 7, 2021·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Clayton T MowrerAngela Hewlett

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