CovR alleviates transcriptional silencing by a nucleoid-associated histone-like protein in Streptococcus mutans.

Journal of Bacteriology
Indranil Biswas, Saswat S Mohapatra

Abstract

In Streptococcus mutans, the global response regulator CovR plays an important role in biofilm formation, stress tolerance response, and caries production. We have previously demonstrated that CovR activates a large gene cluster, which is a part of a genomic island, TnSmu2. In this article, we have further characterized CovR at the molecular level to understand the gene activation mechanism. Toward this end, we mapped the transcription start site of the operon that lies upstream of the SMU.1348 gene (P(SMU.1348)), the first gene of the cluster. We constructed a transcriptional reporter fusion and showed that CovR induces expression from P(SMU.1348). We also demonstrated that purified CovR protects the sequence surrounding the -10 region of P(SMU.1348). In an in vitro transcription assay, we showed that histone-like protein (HLP), a homologue of Escherichia coli HU protein, represses transcription from P(SMU.1348). In vivo overexpression of HLP in trans also represses transcription from P(SMU.1348). Addition of CovR to the HLP-repressed P(SMU.1348) resulted in increased transcription from the promoter, suggesting a role for CovR in countering HLP silencing. Moreover, addition of SMU.1349, a transcriptional activator of the opero...Continue Reading

References

Sep 1, 1987·Microbiological Reviews·K Drlica, J Rouviere-Yaniv
Dec 1, 1986·Microbiological Reviews·W J Loesche
Jun 1, 1980·Microbiological Reviews·S Hamada, H D Slade
Jan 1, 1982·Infection and Immunity·M W Stinson, E J Bergey
Oct 1, 1995·Journal of Bacteriology·C A Nickerson, E C Achberger
Apr 1, 1994·Oral Microbiology and Immunology·R ClaessonJ Carlsson
Jan 1, 1993·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·H K Kuramitsu
Jun 1, 2000·Nature·H OchmanE A Groisman
Aug 31, 2000·Annual Review of Biochemistry·A M StockP N Goudreau
Feb 15, 2001·Current Opinion in Microbiology·M R GrahamJ M Musser
Mar 29, 2002·Molecular Microbiology·Michael J Federle, June R Scott
Oct 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Morag R GrahamJames M Musser
Oct 25, 2002·Proceedings of the National Academy of Sciences of the United States of America·Dragana AjdićJoseph J Ferretti
May 6, 2003·Journal of Bacteriology·Indranil Biswas, June R Scott
Feb 24, 2004·Frontiers in Bioscience : a Journal and Virtual Library·Jeffrey A Banas
Apr 22, 2004·Nature Reviews. Microbiology·Charles J Dorman
Nov 24, 2004·Molecular Microbiology·Marie-Cécile LamyClaire Poyart
Feb 16, 2005·The Journal of Biological Chemistry·Chien-Chung Chen, Hai-Young Wu
Jan 24, 2006·Journal of Bacteriology·Saswati Biswas, Indranil Biswas
Apr 6, 2006·Journal of Bacteriology·Zezhang T WenRobert A Burne
Jun 1, 2006·Microbiology·Janet C Waterhouse, Roy R B Russell
Aug 29, 2006·PLoS Pathogens·Sacha LucchiniJay C D Hinton
Sep 13, 2006·Molecular Biology and Evolution·Pradeep Reddy MarriG Brian Golding
Sep 26, 2006·Journal of Bacteriology·Asiya A GusaJune R Scott
Dec 28, 2006·Nature Reviews. Microbiology·Charles J Dorman
Feb 22, 2007·Oral Microbiology and Immunology·J C WaterhouseR R B Russell
Apr 17, 2007·Nature Structural & Molecular Biology·Emeline BouffartiguesSylvie Rimsky

❮ Previous
Next ❯

Citations

Mar 6, 2018·Microbiology·Pratick KharaIndranil Biswas
Feb 9, 2017·Journal of Bacteriology·Manoharan ShankarIndranil Biswas
Mar 19, 2020·Molecular Microbiology·Saswat S MohapatraEmanuele G Biondi
Apr 7, 2016·Acta Crystallographica. Section F, Structural Biology Communications·Pierce O'NeilIndranil Biswas
Sep 8, 2021·PLoS Genetics·Maria-Vittoria MazzuoliArnaud Firon
Dec 18, 2021·International Journal of Oral Science·Yalan DengTao Hu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.